Phospho-Tyrosine Antibody (216954) [Alexa Fluor® 532]

• Applications: WB, ICC/IF
• Clone: 216954
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Phospho-Tyrosine Antibody (216954) [Alexa Fluor® 532] Summary

• Immunogen: KLH-coupled Phospho-Tyrosine synthetic peptide
• Specificity: Detects proteins containing phosphorylated tyrosine residues in Western blots. In Western blots of pervanadate-treated cell lysates, clone 216954 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.