Peroxidase Activity Assay Kit (Colorimetric)

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24529] - Standard Reference Curve

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24529] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Plant tissue
• Standard Curve Range: 0.5 - 40 U/mL
• Sensitivity: 0.5 U/mL

Peroxidase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Spectrophotometer (240 nm, 420 nm)
  Assay time: 60 min
• Standard Curve Range: 0.5 - 40 U/mL
• Sensitivity: 0.5 U/mL
• Inter-Assay: %CV < 5.5
• Intra-Assay: %CV < 3.8
• Spike Recovery: Average recovery rate: 98%
• Kit Type: Activity Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Peroxidase Activity Assay Kit (Colorimetric)

• EC 1.11.1.7
• Horseradish Peroxidase
• HPRC 1
• HPRC1
• HRPA2
• HRPC 2
• HRPC2
• HRPE5
• HRPN
• Neutral peroxidase
• Peroxidase A2
• Peroxidase C1A
• Peroxidase C1B
• Peroxidase C2
• Peroxidase C3
• Peroxidase E5
• Peroxidase N
• PRXC1A
• PRXC1B
• PRXC2
• PRXC3

Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.