Peroxidase Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-69794
    • Availability
      Product Discontinued

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Peroxidase Antibody Summary

Description
The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product
Immunogen
Highly purified horseradish peroxidase.
Specificity
Soluble Peroxidase immune complex.
Isotype
IgG
Clonality
Polyclonal
Host
Rat
Purity
Ion exchange chromatography
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Applications/Dilutions

Application Notes
.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Alternate Names for Peroxidase Antibody

  • EC 1.11.1.7
  • Horseradish Peroxidase
  • HPRC 1
  • HPRC1
  • HRPA2
  • HRPC 2
  • HRPC2
  • HRPE5
  • HRPN
  • Neutral peroxidase
  • Peroxidase A2
  • Peroxidase C1A
  • Peroxidase C1B
  • Peroxidase C2
  • Peroxidase C3
  • Peroxidase E5
  • Peroxidase N
  • PRXC1A
  • PRXC1B
  • PRXC2
  • PRXC3

Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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