Rat Pepsin ELISA Kit (Colorimetric)

ELISA: Rat Pepsin ELISA Kit (Colorimetric) [NBP2-82494] - Samples were spiked with high concentrations of Rat Pepsin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Pepsin ELISA Kit (Colorimetric) [NBP2-82494] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Rat Pepsin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Pepsin in samples. No Significant cross-reactivity or interference between Rat Pepsin and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.98
• Intra-Assay: CV% < 5.07
• Spike Recovery: 92-110%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Pepsin ELISA Kit (Colorimetric)

• DKFZp666J2410
• ec 3.4.23.1
• Pepsin a precursor
• Pepsinogen 1 second locus
• Pepsinogen 3 group 1
• Pepsinogen 4 group I
• Pepsinogen 5 group I
• Pepsinogen A
• Pepsinogen a3
• Pepsinogen A4
• Pepsinogen A5
• Pepsinogen group 1
• Pepsinogen I
• Pga 3
• Pga 4
• Pga 5
• pga3
• pga4
• pga5

Background

Digestive enzyme with particularly broad specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Pepsin ELISA Kit (NBP2-82494)(2)

Publications using NBP2-82494

• Nagla A El-Shitany, Eman A El-Saidy, Mostafa E El-Naggar, Samia S Sokar Cilostazol protects against gastric ulcers by regulating PPAR-gamma , HO-1, PECAM-1, pErk-1, NF-kappa B, Bcl-2, and cleaved caspase-3 protein expression. Naunyn-Schmiedeberg's archives of pharmacology 2024-06-17 [PMID: 38884677]
• Khalaf HM, Ahmed SM, Welson NN, Abdelzaher WY Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating alpha 7nAChR with inhibiting oxidative stress and apoptosis Journal of biochemical and molecular toxicology 2022-06-15 [PMID: 35702939]

FAQs for Pepsin ELISA Kit (NBP2-82494). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.