Pepsin Antibody

Images

 
Western Blot: Pepsin Antibody [NBP1-51277] - Analysis of Pepsin A (M307) pAb at 1:500 dilutionLane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Western Blot: Pepsin Antibody [NBP1-51277] - Western blot analysis of Pepsin A in K562 cell extracts.

Product Details

Summary
Product Discontinued
View other related Pepsin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51277
    • Availability
      Product Discontinued

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Pepsin Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 281-330 of Human Pepsin A.
Specificity
Pepsin A (M307) pAb detects endogenous levels of Pepsin protein.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-51277 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pepsin Antibody

  • DKFZp666J2410
  • ec 3.4.23.1
  • Pepsin a precursor
  • Pepsinogen 1 second locus
  • Pepsinogen 3 group 1
  • Pepsinogen 4 group I
  • Pepsinogen 5 group I
  • Pepsinogen A
  • Pepsinogen a3
  • Pepsinogen A4
  • Pepsinogen A5
  • Pepsinogen group 1
  • Pepsinogen I
  • Pga 3
  • Pga 4
  • Pga 5
  • pga3
  • pga4
  • pga5

Background

Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Pepsin A is a member of the subfamily A1 within the Pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. Pepsin A is inhibited by ovUS-1, a uterine Serpin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pepsin Antibody (NBP1-51277)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
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Filter By Species
Human
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pepsin Antibody (NBP1-51277). (Showing 1 - 1 of 1 FAQs).

  1. Is the Pepsin antibody NBP1-51277 in lyophilized form?
    • This antibody is not in lyophilized form. It is supplied as 0.1mg in 0.1mL in PBS with sodium azide.

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