Pepsin Antibody

Product Discontinued

Pepsin Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 281-330 of Human Pepsin A.
• Specificity: Pepsin A (M307) pAb detects endogenous levels of Pepsin protein.
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pepsin Antibody

• DKFZp666J2410
• ec 3.4.23.1
• Pepsin a precursor
• Pepsinogen 1 second locus
• Pepsinogen 3 group 1
• Pepsinogen 4 group I
• Pepsinogen 5 group I
• Pepsinogen A
• Pepsinogen a3
• Pepsinogen A4
• Pepsinogen A5
• Pepsinogen group 1
• Pepsinogen I
• Pga 3
• Pga 4
• Pga 5
• pga3
• pga4
• pga5

Background

Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Pepsin A is a member of the subfamily A1 within the Pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. Pepsin A is inhibited by ovUS-1, a uterine Serpin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pepsin Antibody (NBP1-51277)(2)

Publications using NBP1-51277

• Wang J, Yu Z, Ren J et al. Effects of pepsin A on heat shock protein 70 response in laryngopharyngeal reflux patients with chronic rhinosinusitis Acta Otolaryngol. 2017-08-08 [PMID: 28784015] (IHC-P, Human)
• Ren JJ, Zhao Y, Wang J et al. PepsinA as a Marker of Laryngopharyngeal Reflux Detected in Chronic Rhinosinusitis Patients. Otolaryngol Head Neck Surg 2017-05-01 [PMID: 28457223] (WB, Human)

FAQs for Pepsin Antibody (NBP1-51277). (Showing 1 - 1 of 1 FAQs).

1. Is the Pepsin antibody NBP1-51277 in lyophilized form?
   • This antibody is not in lyophilized form. It is supplied as 0.1mg in 0.1mL in PBS with sodium azide.