Peanut Agglutinin [Cy5]

Immunohistochemistry-Paraffin: Peanut Agglutinin [Cy5] [CL-1075-NB] - Tonsil FFPE: Antigen retrieved with Antigen Unmasking Solution and stained with Cy5 labeled Peanut Agglutinin (purple) and DAPI (blue).

Peanut Agglutinin [Cy5] [CL-1075-NB] - Peanut Agglutinin (PNA), Cy5

• Applications: IHC
• Conjugate: Cy5
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Peanut Agglutinin [Cy5] Summary

• Description: Features:br/>
  
• Cy5 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
• Supplied essentially free of unconjugated fluorochromes.
• Excitation: 650 nm
• Emission: 670 nm
• Color: Far Red

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4C, warm to 37C and centrifuge before use. The recommended concentration range for use is 5-20 ug/mL.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, proprietary stabilizer
• Preservative: 0.08% Sodium Azide
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (beta-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide. Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers. A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.