pCLNCX Retrovirus Expression Vector

pCLNCX Retrovirus Expression Vector [NBP2-29502] - Schematic presentation of pCLNCX vector: CMV immediate early promoter/enhancer allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5). For expression under the control of CMV early promoter/enhancer, the gene of interest should be cloned into Hind III or Cla I site.

• Applications: RetVir
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Applications/Dilutions

• Application Notes: The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture. Use in retroviral production reported in scientific literature (PMID: 29121057).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

This expression vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information like the nucleotide sequence.

Alternate Names for pCLNCX Retrovirus Expression Vector

• pCLNCX
• pCLNCX Retrovirus

Background

The pCLNCX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safetymodified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation (1), thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for pCLNCX Retrovirus Expression Vector (NBP2-29502)(6)

Publications using NBP2-29502

• Muir E, Raza M, Ellis C et al. Trafficking and processing of bacterial proteins by mammalian cells: Insights from chondroitinase ABC PLoS ONE. 2017 Nov 27 [PMID: 29121057] (RetVir)
• Keckesova Z, Donaher JL, De Cock J et al. LACTB is a tumour suppressor that modulates lipid metabolism and cell state. Nature Mar 30 2017 [PMID: 28329758] (RetVir)
• Hernandez JL, Davda D, Cheung See Kit M et al. APT2 Inhibition Restores Scribble Localization and S-Palmitoylation in Snail-Transformed Cells Cell Chem Biol. Jan 19 2017 [PMID: 28065656]
• Dean Km, Lubbeck Jl, Davis Lm, Regmi Ck. Microfluidics-Based Selection of Red-Fluorescent Proteins with Decreased Rates of Photobleaching Technical Innovation et al. 2014 Nov 21 [PMID: 25477249]
• Martin BR, Giepmans BN, Adams SR et al. Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nat Biotechnol. 2005 Oct [PMID: 16155565]
• Nishiya N, Tachibana K, Shibanuma M et al. Hic-5-reduced cell spreading on fibronectin: competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase. Mol Cell Biol. 2001 Aug [PMID: 11463817]