pCLNCX Retrovirus Expression Vector

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pCLNCX Retrovirus Expression Vector [NBP2-29502] - Schematic presentation of pCLNCX vector: CMV immediate early promoter/enhancer allows highefficiency transcription in 293 cells. However, this is lost during viral ...read more

Product Details

Summary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

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Applications/Dilutions

Application Notes
The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture. Use in retroviral production reported in scientific literature (PMID: 29121057).
Publications
Read Publications using NBP2-29502.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

This expression vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information like the nucleotide sequence.

Alternate Names for pCLNCX Retrovirus Expression Vector

  • pCLNCX
  • pCLNCX Retrovirus

Background

The pCLNCX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safetymodified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation (1), thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCLNCX Retrovirus Expression Vector (NBP2-29502)(6)

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