pCL-Eco Retrovirus Packaging Vector

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pCL-Eco Retrovirus Packaging Vector [NBP2-29540]

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications NULL
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-Eco Retrovirus Packaging Vector Summary

Specificity
Ecotropic (MoMuLV) for mouse and rat cells only (not human).
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster).
10A1 (MuLV) for most mammalian cells (including hamster).

Applications/Dilutions

Dilutions
  • Retroviral Production reported in scientific literature (PMID 24760698)
Application Notes
The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin
Publications
Read Publications using
NBP2-29540 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24760698). This product can be used in human cells to produce virions to infect non-human cells for protein production.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for pCL-Eco Retrovirus Packaging Vector

  • pCL-Eco Retrovirus
  • pCLECO
  • pCL-Eco

Background

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-Eco Retrovirus Packaging Vector (NBP2-29540)(102)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: RetVir.


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RetVir
(35)
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Human
(23)
Mouse
(9)
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Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using NBP2-29540 Applications Species
Borghesan E, Smith EP, Myeni S Et al. A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication The EMBO journal [PMID: 34423453]
Xu N OPTIMIZING AND UNDERSTANDING CHECKPOINT INHIBITION THERAPY AND CHIMERIC ANTIGEN RECEPTOR THERAPY AGAINST BREAST CANCER Thesis 2020-01-01
Miyauchi S, Kim SS, Jones RN et al. Human papillomavirus E5 suppresses immunity via inhibition of the immunoproteasome and STING pathway Cell reports 2023-05-11 [PMID: 37171962]
Kambarev S, Borghesan E, Miller CN et al. The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth Infection and immunity 2023-04-27 [PMID: 37129527]
Kamimoto K, Adil MT, Jindal K et al. Gene regulatory network reconfiguration in direct lineage reprogramming Stem cell reports 2022-12-26 [PMID: 36584685] (RetVir) RetVir
Wang C, Feng X, Su D et al. Integrated screens uncover a cell surface tumor suppressor gene KIRREL involved in Hippo pathway Proceedings of the National Academy of Sciences 2022-06-21 [PMID: 35704761]
Pease NA, Nguyen PHB, Woodworth MA et al. Tunable, division-independent control of gene activation timing by a polycomb switch Cell reports 2021-03-23 [PMID: 33761349]
Nazari M Plasmonically enhanced photonic inactivation of pathogens Nanoscale 2021-02-04 [PMID: 33538743]
Qing Y, Dong L, Gao L, et al. R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis Molecular cell 2021-01-06 [PMID: 33434505]
Xu N, Palmer DC, Robeson AC, et al. STING agonist promotes CAR T cell trafficking and persistence in breast cancer The Journal of experimental medicine 2021-02-01 [PMID: 33382402]
Show All 102 Publications.

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FAQs for pCL-Eco Retrovirus Packaging Vector (NBP2-29540). (Showing 1 - 4 of 4 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. I'm currently producing a retrovirus with the pCL-Eco packaging vector. However, my target cells are not getting infected, so I was thinking of switching to a lentivirus. Can I use this pCL-Eco packaging vector with a lentivirus, and if so, is it still only infectious to rodents?
    • Unfortunately, we have not yet tested the pCL-Eco vector kit with lentivirus, therefore, we would not be able to provide you with any information regarding this type of vector. I am sorry for any inconvenience.
  3. Can I use your packaging plasmid PCL-Eco Retrovirus packaging vector (pCLNDX Retrovirus expression Vector [NBP2-29540]) with lentiviral vector? I have been using the system of plasmids carrying the gag-pol, rev, GFP-lentivirus and vsv-g (to infect human cells). Can I use the PCL-Eco to infect mouse cells instead of vsv-g?
    • Unfortunately, we have not tested lenti-virus in this packaging kit so I cannot tell you if it will work or not. There are lots of packaging kits on the market for lenti-virus so if you take a look maybe you can find one that will be certain to work. We, however, do not have a lenti-virus kit.
  4. What is the packaging size limitation on Eco Retrovirus Packaging Vector NBP2-29540?
    • The combined size of two inserts should not be more than 4 kb.

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