pCL-Eco Retrovirus Packaging Vector

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pCLNDX Retrovirus Expression Vector [NBP2-29540]

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-Eco Retrovirus Packaging Vector Summary

Specificity
Ectotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Application Notes
The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin Use in Retroviral production reported in scientific literature (PMID 24760698)
Publications
Read Publications using
NBP2-29540 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24760698). This product can be used in human cells to produce virions to infect non-human cells for protein production.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Sequence information for this plasmid is not available. This packaging vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information.

Alternate Names for pCL-Eco Retrovirus Packaging Vector

  • pCL-Eco Retrovirus
  • pCLECO
  • pCL-Eco

Background

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-Eco Retrovirus Packaging Vector (NBP2-29540)(87)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: RetVir.


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RetVir
(32)
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Human
(22)
Mouse
(9)
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Showing Publications 1 - 10 of 87. Show All 87 Publications.
Publications using NBP2-29540 Applications Species
Gan H, Shen T, Chupp DP et al. B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response Sci Adv Apr 1 2020 [PMID: 32270032]
Smith EP, Cotto-Rosario A, Borghesan E et al. Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle mBio Mar 31 2020 [PMID: 32234817]
FernAndez-Messina L, RodrIguez-GalAn A, de YEbenes VG et al. Transfer of extracellular vesicle-microRNA controls germinal center reaction and antibody production EMBO Rep. Feb 19 2020 [PMID: 32073750] (Mouse) Mouse
Woroniecka KI, Rhodin KE, Dechant CA et al. 4-1BB Agonism Averts TIL Exhaustion and Licenses PD-1 Blockade in Glioblastoma and Other Intracranial Cancers Clin. Cancer Res. Dec 23 2019 [PMID: 31871298] (Human) Human
Federman RS, Boguraev AS, Heim EN, DiMaio D Biologically Active Ultra-Simple Proteins Reveal Principles of Transmembrane Domain Interactions J. Mol. Biol. Jul 10 2019 [PMID: 31301406] (Human)

Details:
6 ul was used for each transfection in 293HEKT cells.
Human
Zhu P, Zhang C, Gao Y et al. The transcription factor Slug represses p16Ink4a and regulates murine muscle stem cell aging Nat Commun Jun 12 2019 [PMID: 31189923]
Luong-Gardiol, N;Siddiqui, I;Pizzitola, I;Jeevan-Raj, B;Charmoy, M;Huang, Y;Irmisch, A;Curtet, S;Angelov, GS;Danilo, M;Juilland, M;Bornhauser, B;Thome, M;Hantschel, O;Chalandon, Y;Cazzaniga, G;Bourquin, JP;Huelsken, J;Held, W; Gamma-Catenin-Dependent Signals Maintain BCR-ABL1+ B Cell Acute Lymphoblastic Leukemia Cancer Cell Apr 15 2019 [PMID: 30991025] (RetVir) RetVir
Abraham TS, Flickinger JC, Waldman SA, Snook AE. TCR Retrogenic Mice as a Model To Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen GUCY2C. J. Immunol. Jan 14 2019 [PMID: 30642983] (RetVir) RetVir
Biddy BA, Kong W, Kamimoto K et al. Single-cell mapping of lineage and identity in direct reprogramming Nature Dec 1 2018 [PMID: 30518857] (RetVir, Human) RetVir Human
Suryadevara CM, Desai R, Farber SH et al. Preventing Lck activation in CAR T cells confers Treg resistance but requires 4-1BB signaling for them to persist and treat solid tumors in non-lymphodepleted hosts. Clin. Cancer Res. Nov 13 2018 [PMID: 30425092] (RetVir) RetVir
Show All 87 Publications.

Reviews for pCL-Eco Retrovirus Packaging Vector (NBP2-29540) (0)

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FAQs for pCL-Eco Retrovirus Packaging Vector (NBP2-29540). (Showing 1 - 4 of 4 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. I'm currently producing a retrovirus with the pCL-Eco packaging vector. However, my target cells are not getting infected, so I was thinking of switching to a lentivirus. Can I use this pCL-Eco packaging vector with a lentivirus, and if so, is it still only infectious to rodents?
    • Unfortunately, we have not yet tested the pCL-Eco vector kit with lentivirus, therefore, we would not be able to provide you with any information regarding this type of vector. I am sorry for any inconvenience.
  3. Can I use your packaging plasmid PCL-Eco Retrovirus packaging vector (pCLNDX Retrovirus expression Vector [NBP2-29540]) with lentiviral vector? I have been using the system of plasmids carrying the gag-pol, rev, GFP-lentivirus and vsv-g (to infect human cells). Can I use the PCL-Eco to infect mouse cells instead of vsv-g?
    • Unfortunately, we have not tested lenti-virus in this packaging kit so I cannot tell you if it will work or not. There are lots of packaging kits on the market for lenti-virus so if you take a look maybe you can find one that will be certain to work. We, however, do not have a lenti-virus kit.
  4. What is the packaging size limitation on Eco Retrovirus Packaging Vector NBP2-29540?
    • The combined size of two inserts should not be more than 4 kb.

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