pCL-Eco Retrovirus Packaging Vector

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pCLNDX Retrovirus Expression Vector [NBP2-29540]

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-Eco Retrovirus Packaging Vector Summary

Specificity
Ectotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Application Notes
The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin Use in Retroviral production reported in scientific literature (PMID 24760698)
Publications
Read Publications using
NBP2-29540 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24760698). Human reactivity reported in scientific literature (PMID: 27841869).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Sequence information for this plasmid is not available. This packaging vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information.

Alternate Names for pCL-Eco Retrovirus Packaging Vector

  • pCL-Eco Retrovirus
  • pCLECO
  • pCL-Eco

Background

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-Eco Retrovirus Packaging Vector (NBP2-29540)(73)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: RetVir.


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RetVir
(25)
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Human
(16)
Mouse
(8)
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Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using NBP2-29540 Applications Species
Elia AR, Grioni M, Basso V et al. Targeting tumor vasculature with TNF leads effector T cells to the tumor and enhances therapeutic efficacy of immune checkpoint blockers in combination with ACT. Clin. Cancer Res. Feb 28 2018 [PMID: 29490991] (RetVir) RetVir
Xie X, Deliorman M, Qasaimeh MA, Percipalle P. The relative composition of actin isoforms regulates cell surface biophysical features and cellular behaviors. Biochim. Biophys. Acta. 2018 Feb 02 [PMID: 29410074] (Human) Human
Hones JM, Thivakaran A, Botezatu L et al. Enforced GFI1 expression impedes human and murine leukemic cell growth Sci Rep. 2017 Nov 16 [PMID: 29147018] (RetVir) RetVir
Nazari M, Xi M, Lerch S, Alizadeh MH et al. Plasmonic Enhancement of Selective Photonic Virus Inactivation Sci Rep 2017 Sep 20 [PMID: 28931903] (RetVir) RetVir
He L, Steinocher H, Shelar A, Cohen EB et al. Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions Elife 2017 Sep 04 [PMID: 28869036] (RetVir) RetVir
Park S, Lee S, Lee CG et al. Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5. Nat Commun 2017 Jul 12 [PMID: 28855737] (RetVir, Mouse) RetVir Mouse
Miller CN, Smith EP, Cundiff JA et al. A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication Cell Host Microbe 2017 Aug 23 [PMID: 28844886] (RetVir, Mouse) RetVir Mouse
Karabadzhak AG, Petti LM, Barrera FN et al. Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF ? receptor in an active dimeric conformation Proc. Natl. Acad. Sci. U.S.A. 2017 Aug 29 [PMID: 28808001] (RetVir) RetVir
Kurkewich JL, Hansen J, Klopfenstein N et al. The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis PLoS Genet. 2017 Jul 01 [PMID: 28704388] (RetVir, Mouse) RetVir Mouse
Wang Y, Skibbe JR, Hu C et al. ALOX5 exhibits anti-tumor and drug-sensitizing effects in MLL-rearranged leukemia. Sci Rep May 12 2017 [PMID: 28500307] (Human) Human
Show All 73 Publications.

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FAQs for pCL-Eco Retrovirus Packaging Vector (NBP2-29540). (Showing 1 - 3 of 3 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. I'm currently producing a retrovirus with the pCL-Eco packaging vector. However, my target cells are not getting infected, so I was thinking of switching to a lentivirus. Can I use this pCL-Eco packaging vector with a lentivirus, and if so, is it still only infectious to rodents?
    • Unfortunately, we have not yet tested the pCL-Eco vector kit with lentivirus, therefore, we would not be able to provide you with any information regarding this type of vector. I am sorry for any inconvenience.
  3. Can I use your packaging plasmid PCL-Eco Retrovirus packaging vector (pCLNDX Retrovirus expression Vector [NBP2-29540]) with lentiviral vector? I have been using the system of plasmids carrying the gag-pol, rev, GFP-lentivirus and vsv-g (to infect human cells). Can I use the PCL-Eco to infect mouse cells instead of vsv-g?
    • Unfortunately, we have not tested lenti-virus in this packaging kit so I cannot tell you if it will work or not. There are lots of packaging kits on the market for lenti-virus so if you take a look maybe you can find one that will be certain to work. We, however, do not have a lenti-virus kit.

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