pCL-10A1 Retrovirus Packaging Vector

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Product Details

Summary
Reactivity MaSpecies Glossary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-10A1 Retrovirus Packaging Vector Summary

Specificity
Ectotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Application Notes
AMPLIFICATION: The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml. Use in retroviral production reported in multiple pieces of scientific literature.
Publications
Read Publications using
NBP2-29542 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23973628)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Sequence information for this plasmid is not available. This packaging vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information.

Alternate Names for pCL-10A1 Retrovirus Packaging Vector

  • pCL-10A1 Retrovirus
  • pCL10A1
  • pCL-10A1

Background

The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542)(37)

We have publications tested in 2 confirmed species: Human, Mammal.

We have publications tested in 2 applications: Ret Vir, RetVir.


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Ret Vir
(3)
RetVir
(9)
All Applications
Filter By Species
Human
(14)
Mammal
(1)
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Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NBP2-29542 Applications Species
Sibener LV, Fernandes RA, kolawole EM et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. Jul 26 2018 [PMID: 30053426] (RetVir) RetVir
Johnson CH, Fisher TS, Hoang LT, Felding BH. Luciferase does not alter metabolism in cancer cells. Metabolomics. 2014 Jan 14 (RetVir, Human)

Details:
293T cells were transfected with a mixture of DNA that included pCL0A1
RetVir Human
Gee MH, Han A, Lofgren SM et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell 2017 Dec 20 [PMID: 29275860] (Human) Human
Kodaka M, Xu X, Yang Z et al. Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro Methods Mol. Biol. 2017 Aug 26 [PMID: 28842906] (RetVir, Mammal) RetVir Mammal
Teo Z, Sng MK, Chan JSK et al. Elevation of adenylate energy charge by angiopoietin-like 4 enhances epithelial-mesenchymal transition by inducing 14-3-3Y expression Oncogene 2017 Jul 24 [PMID: 28745316] (RetVir, Human) RetVir Human
Liu H, Dowdle JA, Khurshid S et al. Discovery of Stromal Regulatory Networks that Suppress Ras-Sensitized Epithelial Cell Proliferation. Dev. Cell May 22 2017 [PMID: 28535374]
Bisogno LS, Keene JD. Analysis of post-transcriptional regulation during cancer progression using a donor-derived isogenic model of tumorigenesis. Methods May 19 2017 [PMID: 28529064]
Shinohara K, Yoshida T, Liu H et al. Establishment of novel therapy to reduce progression of myopia in rats with experimental myopia by fibroblast transplantation on sclera. J Tissue Eng Regen Med. Apr 11 2017 [PMID: 28401697]
Zhou Y, Hao Y, Li Y et al. Amplification and up-regulation of MIR30D was associated with disease progression of cervical squamous cell carcinomas. BMC Cancer. Mar 29 2017 [PMID: 28356144]
Bettoun A, Joffre C, Zago G et al. Mitochondrial clearance by the STK38 kinase supports oncogenic Ras-induced cell transformation Oncotarget Jul 12 2016 [PMID: 27283898] (Human) Human
Show All 37 Publications.

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FAQs for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542). (Showing 1 - 2 of 2 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. Can you please let me know if NBP2-29542 contain the Retrovirus ?
    • The pCL-10A1 Retrovirus Packaging Vector NBP2-29542 does not actually contain the retrovirus. The vector is just the DNA plasmid.

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