pCL-10A1 Retrovirus Packaging Vector

Images

 
There are currently no images for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MaSpecies Glossary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-10A1 Retrovirus Packaging Vector Summary

Specificity
Ectotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Application Notes
AMPLIFICATION: The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml. Use in retroviral production reported in multiple pieces of scientific literature.
Publications
Read Publications using
NBP2-29542 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23973628)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Sequence information for this plasmid is not available. This packaging vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29499) for additional information.

Alternate Names for pCL-10A1 Retrovirus Packaging Vector

  • pCL-10A1 Retrovirus
  • pCL10A1
  • pCL-10A1

Background

The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Supplier Logo

Publications for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542)(42)

We have publications tested in 2 confirmed species: Human, Mammal.

We have publications tested in 2 applications: Ret Vir, RetVir.


Filter By Application
Ret Vir
(3)
RetVir
(12)
All Applications
Filter By Species
Human
(14)
Mammal
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NBP2-29542 Applications Species
Martin AP, Jacquemyn M, Lipecka J et al. STK38 kinase acts as XPO1 gatekeeper regulating the nuclear export of autophagy proteins and other cargoes EMBO Rep. Sep 23 2019 [PMID: 31544310]
Johnson CH, Fisher TS, Hoang LT, Felding BH. Luciferase does not alter metabolism in cancer cells. Metabolomics. 2014 Jan 14 (RetVir, Human)

Details:
293T cells were transfected with a mixture of DNA that included pCL0A1
RetVir Human
Oberlick EM, Rees MG, Seashore-Ludlow B et al. Small-Molecule and CRISPR Screening Converge to Reveal Receptor Tyrosine Kinase Dependencies in Pediatric Rhabdoid Tumors Cell Rep Aug 27 2019 [PMID: 31461650]
Pollock, SR;Schinlever, AR;Rohani, A;Kashatus, JA;Kashatus, DF; RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation PLoS ONE Apr 17 2019 [PMID: 30995277] (RetVir) RetVir
Aho ER, Wang J, Gogliotti RD et al. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity Cell Rep Mar 12 2019 [PMID: 30865883] (RetVir) RetVir
Sibener LV, Fernandes RA, kolawole EM et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. Jul 26 2018 [PMID: 30053426] (RetVir) RetVir
Gee MH, Han A, Lofgren SM et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell 2017 Dec 20 [PMID: 29275860] (Human) Human
Durrieu-Gaillard S, Dumay-Odelot H, Boldina G et al. Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements. Cell Cycle Jan 22 2018 [PMID: 29171785] (RetVir) RetVir
Kodaka M, Xu X, Yang Z et al. Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro Methods Mol. Biol. 2017 Aug 26 [PMID: 28842906] (RetVir, Mammal) RetVir Mammal
Teo Z, Sng MK, Chan JSK et al. Elevation of adenylate energy charge by angiopoietin-like 4 enhances epithelial-mesenchymal transition by inducing 14-3-3Y expression Oncogene 2017 Jul 24 [PMID: 28745316] (RetVir, Human) RetVir Human
Show All 42 Publications.

Reviews for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542) (0)

There are no reviews for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542). (Showing 1 - 3 of 3 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. Can you please let me know if NBP2-29542 contain the Retrovirus ?
    • The pCL-10A1 Retrovirus Packaging Vector NBP2-29542 does not actually contain the retrovirus. The vector is just the DNA plasmid.
  3. Can pCL-10A1 Retrovirus Packaging vector (NBP2-29542) be used with Lentivirus shRNA vector pGLV3?
    • When co-transfecting/transducing pCL-10A1 with a pCLX/N expression vector in the acell line, pCL-10A1 will produce retrovial Gag, Pol, and Env proteins for packing the pCLX/N-expressed viral RNA genome.  [pCLX/N expression vectors have both Amp and Neo genes for growing in E.coli and stably transfection in a mammalian cell line, respectively]A key feature for appropriate packaging into viral protein shells is the packaging signal(s)  UR5 on the viral RNA genome, which is derived from one of pCLX/N expression vectors. Check the nucleotide sequence(s) for the packaging signal(s) on your plasmid and compare to established UR5 sequence(s) to asses the likelihood of co-transduction experiments using pCL-10A1 and your vector successfully producing infectious virions.

Other Available Formats

Additional pCL-10A1 Retrovirus Packaging Vector Products

Blogs on pCL-10A1 Retrovirus Packaging Vector

There are no specific blogs for pCL-10A1 Retrovirus Packaging Vector, but you can read our latest blog posts.