PCDHGB2 Antibody (S148-30)

Product Discontinued

PCDHGB2 Antibody (S148-30) Summary

• Immunogen: Fusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma- Protocadherin Gamma B2. Human: 68% identity (60/88 amino acids identical) >60% identity with other Gamma-protocadherin-B proteins
• Localization: Cell Membrane
• Specificity: Greater then 60% identity with other Gamma-protocadherin-B proteins Detects approx 100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDHGB2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Protocadherin Gamma B2 Antibody was sufficient for detection of Protocadherin Gamma B2 in 20 ug of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma B2) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PCDHGB2 Antibody (S148-30)

• MGC126854
• PCDH-GAMMA-B2
• protocadherin gamma subfamily B, 2
• protocadherin gamma-B2

Background

PCDHGB2 is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHGB2
• Uniprot:
  • Q91XX7(Human)