PCDHB6 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC224130 and based on accession number NM_018939. The protein contains a C-terminal DDK tag.
Homo sapiens protocadherin beta 6 (PCDHB6), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for PCDHB6
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for PCDHB6 Overexpression Lysate (Native)
- protocadherin beta 6
- protocadherin beta-6
This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Publications for PCDHB6 Lysate (NBL1-14163) (0)
There are no publications for PCDHB6 Lysate (NBL1-14163).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for PCDHB6 Lysate (NBL1-14163) (0)
There are no reviews for PCDHB6 Lysate (NBL1-14163).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for PCDHB6 Lysate (NBL1-14163) (0)
Additional PCDHB6 Products
Bioinformatics Tool for PCDHB6 Lysate (NBL1-14163)
Discover related pathways, diseases and genes to PCDHB6 Lysate (NBL1-14163). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on PCDHB6