PC-12 Nuclear Cell Lysate

Product Discontinued

PC-12 Nuclear Cell Lysate Summary

• Specificity: PC12 nuclear extracts (Hypoxic and Normoxic) lysate

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Positive control for detection of Hypoxia Inducible Factor by western blotting

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 20mM Hepes (pH 7.9), 100mM KCl, 1mM MgCl2,containing 20% glycerol, protease inhibitors and 0.5 mM DT
• Concentration: 2.5 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Nervous
• Protein State: Native
• Subcellular Fraction: Nuclear

Notes

Before harvesting the cells were deprived of oxygen for 4 hours.

Background

Before harvesting hypoxic cells were deprived of oxygen for 4 hours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for PC-12 Lysate (NB810-55229)(1)

Publication using NB810-55229

• Radek KA, Kovacs EJ, Gallo RL, DiPietro LA. Acute Ethanol Exposure Disrupts VEGF Receptor Cell Signaling in Endothelial Cells. Am J Physiol Heart Circ Physiol. :00699.2007. 2008-05-09 [PMID: 18469146]