Paraquat ELISA Kit (Colorimetric) Summary
| Description |
Paraquat ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of paraquat. |
| Sensitivity |
The actual detection limit is based on the setup of experiment protocol, such as extraction procedure and dilution factor. |
| Assay Type |
Competitive ELISA |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
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Components
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- 96-wells microtiter plate (#S): Twelve strips of 8 detachable wells coated withAnti-Paraquat antibody.
- Calibrators: Containing of 0, 0.75, 2.5 and 7.5 ng/mL of Paraquat.
- Paraquat-Horseradish Peroxidase Conjugate (PRQ-HRP) (#3)
- Stabilized tetramethylbenzidine (TMB) substrate (#5): Ready to use.
- Stop Solution (#7): 3 N HCl.
- Wash Buffer (10x PBS-Tween) (#6): Dilute 10 fold with distilled or deionizedwater to 150 mL prior to use.
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Paraquat ELISA Kit (Colorimetric)
Background
Paraquat is the trade name for N,N'-Dimethyl-4,4'-bipyridinium dichloride, a viologen and is today among the most commonly used herbicides. Paraquat is used as a quaternary ammonium herbicide. It is toxic to humans if ingested. Other members of this class include diquat, cyperquat, diethamquat, difenzoquat, and morfamquat. All of these are easily reduced to the radical ion, which generates superoxide radical that reacts with unsaturated membrane lipids. As a herbicide the compound is quick-acting, non-selective, and kills green plant tissue on contact. It is redistributed within the plant but does not harm mature bark. Paraquat protects crops by controlling a wide range of annual and certain perennial weeds (unwanted plants) that reduce crop yield and quality by competing with the crop for water, nutrients, and light. The key characteristics that distinguish the non-selective contact herbicide paraquat from other active ingredients used in plant protection products are: It is non-selective, which means it kills a wide range of annual grasses and broad-leaved weeds and the tops of established perennial weeds. It is very fast-acting. It is rain-fast within minutes of application. It becomes biologically inactive upon contact with soil.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for Paraquat ELISA Kit (KA1424)(3)
Showing Publications 1 -
3 of 3.
| Publications using KA1424 |
Applications |
Species |
| Q Li, H Xiao, Y Shao, X Chang, Y Zhang, Z Zhou Paraquat increases Interleukin-1&beta in hippocampal dentate gyrus to impair hippocampal neurogenesis in adult mice Ecotoxicol. Environ. Saf., 2020-05-22;200(0):110733. 2020-05-22 [PMID: 32450442] |
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| Camacho-Morales RL, Guillen-Navarro K, Sanchez JE. et al. Degradation of the herbicide paraquat by macromycetes isolated from southeastern Mexico. Biotech 2017-09-16 [PMID: 28955621] |
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| Wen X, Gibson CJ, Yang I et al. MDR1 Transporter Protects Against Paraquat-Induced Toxicity in Human and Mouse Proximal Tubule Cells. Toxicol Sci. 2014-07-11 [PMID: 25015657] |
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Product General Protocols
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FAQs for Paraquat ELISA Kit (KA1424). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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