Parainfluenza Virus 2 Antibody (PIV21)

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Product Details

Summary
Reactivity ViSpecies Glossary
Applications ELISA, ICC/IF
Clone
PIV21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Parainfluenza Virus 2 Antibody (PIV21) Summary

Immunogen
MA 104 cells, inoculated with parainfluenza 2 virus, strain II ALTB cc 2056. Virus was grown on monolayer of MA cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (5000 rpm, 40 min). Virus was pelleted by high speed centrifugation at 18000 rpm for 2.5 hours in the SW27 rotor for 20 min at + 4 oC, then the supernatant was collected and protein concentration was determined by Lowry method.
Specificity
This reacts with F protein of parainfluenza virus type 2. Purity > 90 % (SDS-PAGE)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ELISA: Use at an assay dependent concentration. I-ELISA: Use at an assay dependent dilution (using an antimouse HRP conjugate on second stage).IF: Use at an assay dependent concentration. Recommended for preparation of HRP conjugates for detection of parainfluenza type 2 virus.

Reactivity Notes

This antibody is reactive to Parainfluenze virus 2, but not type 1 or type 3.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Parainfluenza Virus 2 Antibody (NB120-8355) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-8355AF405
Alexa Fluor 488 Labeled NB120-8355AF488
Alexa Fluor 647 Labeled NB120-8355AF647
Alexa Fluor 700 Labeled NB120-8355AF700
DyLight 350 Labeled NB120-8355UV
DyLight 405 Labeled NB120-8355V
DyLight 488 Labeled NB120-8355G
DyLight 680 Labeled NB120-8355FR
DyLight 755 Labeled NB120-8355IR

Additional Parainfluenza Virus 2 Products

Parainfluenza Virus 2 NB120-8355

Blogs on Parainfluenza Virus 2

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