PAP Pen Fine Tip

• Applications: Func, ICC/IF, IHC

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • In-situ Hybridization
• Application Notes: Preparation of Working Solutions
  1. Ready to be used directly on wet slides.
  
  Protocol Recommendations
  1. Hold the pen point up and press it with your fingertip to take blocked air out from the pen barrel to prevent overflow.
  2. To start, press the tip of the pen straight down until fluid starts soaking the tip and then draw a circle around the specimen.

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

Elite PAP pen provides a thin, film-like water repellent barrier when a circle is drawn around the specimen on the glass slide. This barrier creates the proper surface tension to hold antibody solution within the target area on the slide. This precludes the need for wiping around the specimen during the staining procedure. Since the reagent is guarded by the hydrophobic circle around the tissue, it minimizes the undesirable dilution of reagents with the wash buffer. This pen can be used for PAP, ABC, immunofluorescence, frozen sections and in situ hybridization procedures. The Mini PAP pen is especially useful when working with multiple sections on a single slide because its tip is finer than the regular PAP pen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.