Pancreatic Islet Lysate Antibody

• Reactivity: Po
• Applications: WB, S-ELISA
• Clonality: Polyclonal
• Host: Canine
• Conjugate: Unconjugated

Pancreatic Islet Lysate Antibody Summary

• Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells
• Isotype: IgG
• Clonality: Polyclonal
• Host: Canine
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:50-1:200
  • Sandwich ELISA 1:50 -1:200
• Application Notes: This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) NBP1-73281and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) NBP1-73281 and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: IgG purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.