Pancreatic Islet Lysate Antibody

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, S-ELISA
Clonality
Polyclonal
Host
Canine
Conjugate
Unconjugated

Order Details

Pancreatic Islet Lysate Antibody Summary

Immunogen
Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells
Isotype
IgG
Clonality
Polyclonal
Host
Canine
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:50-1:200
  • Sandwich ELISA 1:50 -1:200
Application Notes
This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) NBP1-73281and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) NBP1-73281 and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
IgG purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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