Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, IHC-Fr, WB (-)
Clone
HIS52
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) Summary

Immunogen
The immunogen for this antibody is Pan endothelium.
Modification
p Ser123, p Ser124
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1-10 ug/ml
Application Notes
The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections. The HIS52 antibody has been reported for use in flow cytometric analysis. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations. is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-00076AF350
Alexa Fluor 405 NBP2-00076AF405
Alexa Fluor 488 NBP2-00076AF488
Alexa Fluor 532 NBP2-00076AF532
Alexa Fluor 594 NBP2-00076AF594
Alexa Fluor 647 NBP2-00076AF647
Alexa Fluor 700 NBP2-00076AF700
Alexa Fluor 750 NBP2-00076AF750
Allophycocyanin NBP2-00076APC
Allophycocyanin/Cy7 NBP2-00076APCCY7
DyLight 350 NBP2-00076UV
DyLight 405 NBP2-00076V
DyLight 488 NBP2-00076G
DyLight 550 NBP2-00076R
DyLight 594 NBP2-00076DL594
DyLight 650 NBP2-00076C
DyLight 680 NBP2-00076FR
DyLight 755 NBP2-00076IR
Janelia Fluor 549 NBP2-00076JF549
Janelia Fluor 646 NBP2-00076JF646
PE NBP2-00076PE
PE/Atto594 NBP2-00076PEATT594
PE/Cy5.5 NBP2-00076PECY55
PE/Cy7 NBP2-00076PECY7
PerCP NBP2-00076PCP

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