Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) - BSA Free

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00076
    • Availability
      Product Discontinued

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Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) - BSA Free Summary

Immunogen
The immunogen for this antibody is Pan endothelium.
Modification
p Ser123, p Ser124
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1-10 ug/ml
Application Notes
The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections. The HIS52 antibody has been reported for use in flow cytometric analysis. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations. is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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