Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52)

• Reactivity: Rt
• Applications: Flow, IHC, IHC-Fr, WB (-)
• Clone: HIS52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) Summary

• Immunogen: The immunogen for this antibody is Pan endothelium.
• Modification: p Ser123, p Ser124
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1-10 ug/ml
• Application Notes: The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections. The HIS52 antibody has been reported for use in flow cytometric analysis. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations. is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Background

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.