Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, IHC-Fr, WB (-)
Clone
HIS52
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) Summary

Immunogen
The immunogen for this antibody is Pan endothelium.
Modification
p Ser123, p Ser124
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1-10 ug/ml
Application Notes
The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections. The HIS52 antibody has been reported for use in flow cytometric analysis. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations. is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-00076AF405
Alexa Fluor 488 Labeled NBP2-00076AF488
Alexa Fluor 647 Labeled NBP2-00076AF647
Alexa Fluor 700 Labeled NBP2-00076AF700
Allophycocyanin Labeled NBP2-00076APC
DyLight 350 Labeled NBP2-00076UV
DyLight 405 Labeled NBP2-00076V
DyLight 488 Labeled NBP2-00076G
DyLight 680 Labeled NBP2-00076FR
DyLight 755 Labeled NBP2-00076IR
PE Labeled NBP2-00076PE
PerCP Labeled NBP2-00076PCP

Additional Pan-Endothelium Marker Products

Pan-Endothelium Marker NBP2-00076

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