Human, Mouse, Rat p38 Pathway Assay ELISA Kit

Product Discontinued

Human, Mouse, Rat p38 Pathway Assay ELISA Kit Summary

• Specificity: Kit contains (1) 96 well strip plate of p38 alpha (pT180/Y182) 6 pairs of strips (12 strips total) with a kit assay control for the qualitative detection of phosphorylated p38 alpha protein in tissue lysates, cell lysates and recombinant protein preparations.
• Kit Type: ELISA Kit

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This ELISA product range provides a quick, easy, more sensitive, and qualitatively better alternative to western blotting for kinase assay readouts, and it is designed to demonstrate the qualitative differences in the amounts of specific target proteins between samples.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

You may also Mix-and-Match you own Pathway Assay Kit by purchasing catalog number NBP1-71668. Choose up to 6 different targets from this list: Active Beta Catenin (DP S33/S37/T41), AKT1 (pS473), AKT2 (pS474), CREB (pS133), ERK1/2 (pT202/Y204; pT185/Y187), GSK3 alpha (pS21), GSK3 beta (pS9), p38 (pT180/Y182).

View All Available Pathway Assay Kits

Background

Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for p38 Pathway Assay ELISA Kit (NBP1-71674). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.