Oxidized PTP Active Site Antibody (335636) [Alexa Fluor® 594]

• Reactivity: All-Multi
• Applications: WB
• Clone: 335636
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Oxidized PTP Active Site Antibody (335636) [Alexa Fluor® 594] Summary

• Immunogen: KLH-coupled perfomic acid oxidized synthetic peptide
  VHCSAG
• Specificity: Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oxidized PTP Active Site Antibody (335636) [Alexa Fluor® 594]

• Oxidized PTP Active Site

Background

Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) by exogenous or intracellular oxidants such as hydrogen peroxide is believed to play an important role in regulating the activity of these enzymes. The initial oxidation to a sulfenic acid (-SOH) is reversible, but the oxidation often progresses to an irreversible sulfonic acid (-SO₃H) that can be detected by modification-specific antibodies. The active sites of PTPs contain the consensus amino acid sequence (V/I)HCSXG, a sequence essential for catalytic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.