Ovalbumin Antibody (6G2) - BSA Free

Western Blot: Ovalbumin Antibody (6G2) - BSA Free [NBP1-05137] - Review image from a confirmed customer using anti-OVA antibody on Bacterial lysates (WT or bacteria containing expression plasmid for cytoplasmic cOVA) and chicken ovalbumin (Sigma)

Product Discontinued

Ovalbumin Antibody (6G2) - BSA Free Summary

• Immunogen: Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35C and the autoclaved for 1 h at 110C (2) before being adsorbed onto aluminum hydroxide gel.
• Specificity: Reacts with denatured or modified forms (surface-adsorbed, heat-denature or reduced) of ovalbumin. Cross reactivity with ovalbumins from other avian species has not been tested.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OVAL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:12000

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ovalbumin Antibody (6G2) - BSA Free

• Allergen Gal d 2
• CAP 2
• CAP2
• Cytoplasmic antiproteinase 2
• Egg albumin
• Gal d II
• Hen egg white
• PI8
• Plakalbumin
• Protease inhibitor 8
• Serpin B8
• serpin peptidase inhibitor clade B member 8
• SERPINB14
• SERPINB8

Background

Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbumin is a globular monomer with a molecular mass of 45 kDa (1). It is structurally related to both serpins and lipocalins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Ovalbumin Antibody (NBP1-05137) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anony- mous WB Other 11/25/2013

Summary

• Application: Western Blot
• Sample Tested: Bacterial lysates (WT or bacteria containing expression plasmid for cytoplasmic cOVA) and chicken ovalbumin (Sigma)
• Species: Other
• Comments: Results: Ovalbumin positive control works as expected (band at ~45kDa). Bacterial lysates suggest bacteria are unsuccessful in expressing cOVA with the present conditions or antibody is unable to recognize the truncated cOVA (first 45 Amino Acids removed for cOVA). Unfortunately because of the experimental conditions I am not able to determine if the antibody recognizes the truncated cOVA protein.

Blocking

• Blocking Details: Boiled 5min Laemmeli buffer, loaded 12%gel to PVDF TransBlotTurbo(BioRad) wash w/ TBS-T, blocked w/ 5%BSA/TBS-T 1hr RT

Primary Anitbody

• Dilution Ratio: Anti-OVA was diluted 1:5000 in 5%BSA/TBS-T incubated at 4C ON (16h) w/ mild agitation on shaker washed 3X 10 min w/ TBS-T

Secondary Antibody

• Secondary Description: Jackson Immunoresearch anti-mouse HRP
• Secondary Concentration: 1:10000 with streptavidin and 1:50000 for chemiluminescent visualization of ladder all in 10 mL 5%BSA/TBS-T

Details

• Detection Notes: Secondary incubated 1h at RT with rocking. TBS-T used 4X washes 15min each at RT. Blots were visualized on a BioRad ChemiDoc XRS

Comments

• Comments: Results: Ovalbumin positive control works as expected (band at ~45kDa). Bacterial lysates suggest bacteria are unsuccessful in expressing cOVA with the present conditions or antibody is unable to recognize the truncated cOVA (first 45 Amino Acids removed for cOVA). Unfortunately because of the experimental conditions I am not able to determine if the antibody recognizes the truncated cOVA protein.

Bioinformatics

• Gene Symbol: OVAL