Ovalbumin Antibody (6G2) - BSA Free

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Western Blot: Ovalbumin Antibody (6G2) - BSA Free [NBP1-05137] - Review image from a confirmed customer using anti-OVA antibody on Bacterial lysates (WT or bacteria containing expression plasmid for cytoplasmic cOVA) ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-05137
    • Availability
      Product Discontinued

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Ovalbumin Antibody (6G2) - BSA Free Summary

Immunogen
Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35C and the autoclaved for 1 h at 110C (2) before being adsorbed onto aluminum hydroxide gel.
Specificity
Reacts with denatured or modified forms (surface-adsorbed, heat-denature or reduced) of ovalbumin. Cross reactivity with ovalbumins from other avian species has not been tested.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OVAL
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:12000
Reviewed Applications
Read 1 Review rated 4
using
NBP1-05137 in the following applications:

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Ovalbumin Antibody (6G2) - BSA Free

  • Allergen Gal d 2
  • CAP 2
  • CAP2
  • Cytoplasmic antiproteinase 2
  • Egg albumin
  • Gal d II
  • Hen egg white
  • PI8
  • Plakalbumin
  • Protease inhibitor 8
  • Serpin B8
  • serpin peptidase inhibitor clade B member 8
  • SERPINB14
  • SERPINB8

Background

Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbumin is a globular monomer with a molecular mass of 45 kDa (1). It is structurally related to both serpins and lipocalins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Ovalbumin Antibody (NBP1-05137) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-05137:
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Western Blot Ovalbumin NBP1-05137
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4
reviewed by:
Anony- mous
WB Other 11/25/2013
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Summary

ApplicationWestern Blot
Sample TestedBacterial lysates (WT or bacteria containing expression plasmid for cytoplasmic cOVA) and chicken ovalbumin (Sigma)
SpeciesOther
CommentsResults: Ovalbumin positive control works as expected (band at ~45kDa). Bacterial lysates suggest bacteria are unsuccessful in expressing cOVA with the present conditions or antibody is unable to recognize the truncated cOVA (first 45 Amino Acids removed for cOVA). Unfortunately because of the experimental conditions I am not able to determine if the antibody recognizes the truncated cOVA protein.

Blocking

Blocking DetailsBoiled 5min Laemmeli buffer, loaded 12%gel to PVDF TransBlotTurbo(BioRad) wash w/ TBS-T, blocked w/ 5%BSA/TBS-T 1hr RT

Primary Anitbody

Dilution RatioAnti-OVA was diluted 1:5000 in 5%BSA/TBS-T incubated at 4C ON (16h) w/ mild agitation on shaker washed 3X 10 min w/ TBS-T

Secondary Antibody

Secondary DescriptionJackson Immunoresearch anti-mouse HRP
Secondary Concentration1:10000 with streptavidin and 1:50000 for chemiluminescent visualization of ladder all in 10 mL 5%BSA/TBS-T

Details

Detection NotesSecondary incubated 1h at RT with rocking. TBS-T used 4X washes 15min each at RT. Blots were visualized on a BioRad ChemiDoc XRS

Comments

CommentsResults: Ovalbumin positive control works as expected (band at ~45kDa). Bacterial lysates suggest bacteria are unsuccessful in expressing cOVA with the present conditions or antibody is unable to recognize the truncated cOVA (first 45 Amino Acids removed for cOVA). Unfortunately because of the experimental conditions I am not able to determine if the antibody recognizes the truncated cOVA protein.

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Recent Reviews

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Anony- mous
11/25/2013
Application: WB
Species: Other

Bioinformatics

Gene Symbol OVAL