ORF8 Antibody (1041422) [Alexa Fluor™ Plus 680]

• Reactivity: V
• Clone: 1041422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

ORF8 Antibody (1041422) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human SARS-CoV-2 ORF8 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ORF8 Antibody (1041422) [Alexa Fluor™ Plus 680]

• ORF8

Background

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). M protein is the most abundant structural protein in the coronavirus membrane, and it is predicted to span the membrane three times, with a short N-terminal domain outside the viral envelope and a long C-terminal domain inside the virion (2). SARS-CoV-2 M protein is a 222 amino acid (aa) glycoprotein that is composed of an 18 aa N-terminal domain on the viral surface, 3 transmembrane domains, and a 122 aa C-terminal domain inside the viral envelope. SARS-CoV-2 M protein shares 89.14%, 98.6%, 98.2%, and 38.36% aa similarity with SARS-CoV-1, bat SARS-CoV, pangolin SARS-CoV, and MERS-CoV M proteins, respectively (3). The M protein of coronavirus plays an important role in assembly of viral particles by interacting with other structural proteins, especially with the E protein (4, 5). In SARS-CoV-2, M protein, combined with E protein, regulates intracellular trafficking of the S Protein and its unique furin-mediated processing (6).

1. Wu, F. et al. (2020) Nature 579:265.
2. Mousavizadeh, L. and S. Ghasemi (2020) J. Microbiol. Immunol. Infect. doi:10.1016/j.jmii.2020.03.022.
3. Thomas, S. (2020) Pathog. Immun. 5:342.
4. Masters, P.S. (2006) Adv. Virus. Res. 66:193.
5. Corse, E. and C.E. Machamer (2003) Virology 312:25.
6. Boson, B. et al. (2020) bioRxiv doi:10.1101/2020.08.24.260901.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.