ORF73/HHV8 Antibody (4C11) - BSA Free

Western Blot: ORF73/HHV8 Antibody (4C11) [NBP1-30176] - The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-30176. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate (40ug). Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)

Immunocytochemistry/Immunofluorescence: ORF73/HHV8 Antibody (4C11) [NBP1-30176] - Hyperphosphorylation of Akt induced by KSHV (ORF73/HHV8) in THP-1 infected cells is resistant to Bortezomib treatment.Immunofluorescence of mock and KSHV-infected THP-1 cells with anti-LANA antibodies. Typical LANA staining (intranuclear red punctuation) is visible in cells latently infected by KSHV. The counterstaining of THP-1 DNA with DAPI (blue) is shown. Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/1756-9966-32-79) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: ORF73/HHV8 Antibody (4C11) [NBP1-30176] - IHC analysis of Kaposi's sarcoma tissue using antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

• Reactivity: V-Vi
• Applications: WB, ELISA, IHC
• Clone: 4C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ORF73/HHV8 Antibody (4C11) - BSA Free Summary

• Immunogen: Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli (AAB62657).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HHV8GK18_gp81
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-1:2000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24422998)

Reactivity Notes

Human herpesvirus 8 (HHV-8) (Kaposi's sarcoma-associated herpesvirus)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ORF73/HHV8 Antibody (4C11) - BSA Free

• HHV8GK18_gp81
• LANA
• LANA1
• LNA
• ORF73

Background

ORF73 protein of human herpesvirus 8(HHV-8) is a Kaposi's sarcoma-associated herpesvirus (KSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ORF73/HHV8 Antibody (NBP1-30176)(5)

Publications using NBP1-30176

• Moodad S, El Hajj R, Hleihel R et al Lenalidomide in Combination with Arsenic Trioxide: an Effective Therapy for Primary Effusion Lymphoma Cancers (Basel) 2020-09-05 [PMID: 32883022] (WB, WB)
  
  Details:
  Citation using the HRP version of this antibody.
• Kwun HJ, Toptan T, Ramos da Silva S et al. Human DNA tumor viruses generate alternative reading frame proteins through repeat sequence recoding. Proc. Natl. Acad. Sci. U.S.A. 2014-10-14 [PMID: 25271323] (WB)
• Gonnella R, Santarelli R, Farina A et al. Kaposi sarcoma associated herpesvirus (KSHV) induces AKT hyperphosphorylation, bortezomib-resistance and GLUT-1 plasma membrane exposure in THP-1 monocytic cell line Journal of Experimental & Clinical Cancer Research 2013-12-01 [PMID: 24422998] (Block/Neutralize, Immunocytochemistry/ Immunofluorescence)
• Gonnella R, Santarelli R, Farina A et al. Kaposi sarcoma associated herpesvirus (KSHV) induces AKT hyperphosphorylation, bortezomib-resistance and GLUT-1 plasma membrane exposure in THP-1 monocytic cell line. J Exp Clin Cancer Res 2013 Oct 23 [PMID: 24422998] (ICC/IF, Human)
• Moodad SH VIRALLY-INDUCED HEMATOLOGICAL MALIGNANCIES WITH DISMAL PROGNOSIS: IMPACT OF EPIGENETICS AND POTENTIAL THERAPEUTICS Thesis 2021-01-01 (WB)

FAQs for ORF73/HHV8 Antibody (NBP1-30176). (Showing 1 - 3 of 3 FAQs).

1. I need to do Viral IL-6 (HHV8) ELISA. I was wondering if you have the kit if not would it be possible to make it for us? I would appreciate if you inform me with all the details.
   • We do not have an HHV8 kit, but we have two HHV8 antibodies that may be used for an ELISA: NBP1-30176 and NBP1-47357.
2. Why does the image for WB shows a band at 75 kDa, when the molecular weight for ORF73 is actually a lot higher? Do you have a different image for WB or any other information regarding this?
   • The Western blot image on our site was generated using a cell lysate from 293T cells transfected with the partial recombinant protein used to generate the antibody. That is, the image on the site is not for a Western blot against the full-length protein. This is why it shows up at a lower molecular weight.
3. I have conjugated the ORF 73/HHV8 4C11 antibody (NBP1-30176) using an AlexaFluor 647 Antibody Labeling Kit. All absorbance calculations indicate that the labeling was successful. However, upon attempting IF with the newly conjugated antibody, it no longer
   • For product NBP1-30176, we have not yet tried conjugating this antibody to a fluor or other dye. Depending on the labeling chemistry, it is possible that the fluor is binding or blocking the antigen binding region of the antibody. Since you were not able to detect any signal when using a conjugated secondary with the directly conjugated product, this would suggest that the conjugated antibody is not binding to your sample. With our conjugations, we typically use a free amine labeling chemistry to bind our fluors to the antibodies. We have, in some cases, found that the antibody binding efficiency has been compromised by this labeling. In such cases, we resort to using a conjugated secondary for detection. Therefore, I do not have any additional troubleshooting advice to offer in this case. I am sorry for any inconvenience.

Bioinformatics

• Gene Symbol: HHV8GK18_gp81
• Uniprot:
  • O40947(Human)