ORANGE Peroxidase Substrate Kit

Immunohistochemistry: ORANGE Peroxidase Assay Kit [KA0563] - Staining of class III beta-tubulin in retinal pigment epithelial cells using chromogen ORANGE Peroxidase Assay Kit KA0563

Product Discontinued

ORANGE Peroxidase Substrate Kit Summary

• Description: Quality control test: IHC demonstration data
  
  Materials Required but Not Supplied: Primary antibody; Biotin-labeled secondary antibody, HRP-labeled streptavidin and serum block; Isopropyl alcohol; Mounting media (aqueous or xylene-based); 0.1 M Tris-HCl or PBS (See Reagent Preparation). Metal enhanced DAB technology permits detection of extremely low concetrations of tissue antigens. This kit provides a sensitive means for detecting peroxidase labeled antibodies and conjugates. ORANGE Peroxidase Substrate Kit contains nickel enhanced DAB that produces an orange-red DAB polymer and also exceeds the sensitivity of DAB alone.
• Kit Type: Substrate Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ORANGE Peroxidase Substrate Kit

• DAB

Background

The application of antibodies and other proteins covalently coupled to horseradish peroxidase (HRP) in immunohistology is well documented. It is the most frequently used label for immunohistologic techniques. In the presence of peroxide (H2O2), HRP catalyzes the oxidation of phenols, naphthols, diamines, aminophenols, indophenols etc. forming chromogenic products visible by light microscopy. Most commonly employed are 3-amino-9-ethylcarbazole, p-phenylenediamine/catechol, 4-chloro-1-naphthol and diaminobenzidine (DAB). Although a suspected carcinogen, DAB is the most widely accepted donor substrate for peroxidase immunohistochemistry, since it provides a reaction product insoluble in alcohols and xylene. The DAB oxidation product is light brown and may be difficult to visualize if low antigen density occurs. The ORANGE Substrate System is based on oxidative coupling of aromatic amines and phenolic compounds by HRP in the presence of peroxides. Endogenous peroxidase activity is eliminated by controlled oxidation prior to application of antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for ORANGE Peroxidase Kit (KA0563). (Showing 1 - 1 of 1 FAQ).

1. Does Novus have some multistaining kits for IHC?. Our customer needs to stain his IHC with with different reactivities in the same tissue and he is looking for kits with this function.
   • For the staining of multiple antigens, your customer may employ immunoenzymatic or immunofluorescence staining methods. For immunoenzymatic staining, we offer several chromogen, and you may suggest the following list of kits to the customer:, Reliable DAB Peroxidase Assay Kit 500 Slides (KA0562), RED Phosphatase Assay Kit 1000 Slides (KA0566), Real Blue Peroxidase Assay Kit 500 Slides (KA0565), ORANGE Peroxidase Assay Kit 1000 Slides (KA0563), BLUE Phosphatase Assay Kit 1000 Slides (KA0567). For immunofluorescence staining, we offer several types of secondary antibodies (conjugated to different immunofluorescent dyes) which may be used for labeling different antigens. The customer may chose the options from our secondary antibodies.