OR2B6 Overexpression Lysate

Product Discontinued

OR2B6 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_036499. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens OR2B6, mRNA.
• Gene: OR2B6

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OR2B6 Overexpression Lysate

• dJ408B20.2
• hs6M1-32
• MGC120190
• MGC129828
• Olfactory receptor 2B1
• Olfactory receptor 2B5
• olfactory receptor 2B6
• Olfactory receptor 5-40
• Olfactory receptor 6-31
• Olfactory receptor OR6-4
• olfactory receptor, family 2, subfamily B, member 1 pseudogene
• olfactory receptor, family 2, subfamily B, member 5
• olfactory receptor, family 2, subfamily B, member 6 pseudogene
• olfactory receptor, family 2, subfamily B, member 6
• OR2B1
• OR2B1P
• OR5-40OR2B5
• OR5-41
• OR6-31OR2B6P

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: OR2B6