Oligodendrocyte Marker O4 Antibody (O4) [NL637]

BM-NSCs OLGs differentiation in vitro and transplantation in Shi mice. (A) BM-NSCs were cultured in oligodendrocyte differentiation media for 48 h, 1 week, and 2 weeks for O4 (differentiation marker) and Ki-67 (proliferation marker) staining. (B) Fluorescence staining of BM-NSCs (mCherry+ cells), after 35 days post injection, show myelination (MBP+) in the contralateral striatum. Scale bar = 50 μm. (C) Quantification of tremor of Shi mice treated with OPCs. (D) Transplanted OPCs prolong the survival of Shi mice compared with PBS-treated (n = 9). Symbols represent mean ± SD. ∗p < 0.05, Student’s t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31231194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fundamental steps of BM-NSCs OLGs differentiation. (A) Expression of Ki-67 at day 38, as shown by immunofluorescence analysis. (B,C) Immunofluorescence staining of progenitor cells at day 38, expressing A2B5, and NG2. (D,E) RIP and O4 staining show cells ramified morphology. (F) Expression of Ki-67 which in late stage of OLGs differentiation (day 52). (G) At the end of differentiation stage, CNPase+ OLGs show typical ramified morphology. (H) OPCs and OLGs proliferation (A,F) was quantified as percentage of Ki67-positive cells. Scale bar = 50 μm. (I) The percentages of A2B5+, NG2+, RIP+, O4+, and CNPase+ cells are present in EOLG+LOLG-DM cultures as compared to OPC-PM cultures. Data are expressed as the mean ± SD, three independent experiments, ∗∗p < 0.01, Student’s t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31231194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Discontinued

Oligodendrocyte Marker O4 Antibody (O4) [NL637] Summary

• Additional Information: Clone UCHT1 was used by HLDA to establish CD designation
• Specificity: Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

Publications

Read Publication using NL1326V in the following applications: ICC 1 publication

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O4 Antibody (O4) [NL637]

• Oligodendrocyte Marker O4

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
4. Gard, A. et al. (1995) Dev. Biol. 167:596.
5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
8. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O4 Antibody (NL1326V)(1)

Publication using NL1326V

• MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018-06-05;23(10):2864-2873.e7. 2018-06-05 [PMID: 29874574] (ICC, Mouse)