Oligodendrocyte Marker O4 Antibody (O4) [Allophycocyanin]

Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 APC-conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow
• Clone: O4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Oligodendrocyte Marker O4 Antibody (O4) [Allophycocyanin] Summary

• Immunogen: Bovine brain corpus callosum white matter
• Specificity: Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O4 Antibody (O4) [Allophycocyanin]

• Oligodendrocyte Marker O4

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
4. Gard, A. et al. (1995) Dev. Biol. 167:596.
5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
8. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O4 Antibody (FAB1326A)(1)

Publication using FAB1326A

• AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church A comprehensive library of human transcription factors for cell fate engineering Nature Biotechnology, 2020-11-30;0(0):. 2020-11-30 [PMID: 33257861] (Flow Cytometry, Mouse)

Review for Oligodendrocyte Marker O4 Antibody (FAB1326A) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Nuria Álvarez-Sánchez Flow Mouse 02/07/2019

Summary

• Application: Flow Cytometry
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: ADKY0216111

Comments

• Comments: Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for MOG, O4, GalC and AN2, plus for viability to exclude dead cells. MOG antibody was biotinylated, so cells were subsequently incubated with streptavidin-BV786 to stain MOG+ cells. Viable cells were gated and O4 staining is showed in non-stained cells (blue histogram) and in stained cells (red histogram).