![Western Blot: O-GlcNAc Antibody (9H6) [NBP2-59385] - Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-9H6-Western-Blot-NBP2-59385-img0005.jpg "Western Blot: O-GlcNAc Antibody (9H6) [NBP2-59385] - Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 9H6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Synthetic B-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | Specific for B-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins. |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | PEG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for O-GlcNAc Antibody (NBP2-59385)Find related products by research area.
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O-GlcNAc, Glucose Deprivation and Cancer O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-9H6-Immunocytochemistry-Immunofluorescence-NBP2-59385-img0006.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.")
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-9H6-Immunocytochemistry-Immunofluorescence-NBP2-59385-img0002.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.")
