NusA Antibody (A6-E7)

Western Blot: NusA Antibody (A6-E7) [NBP2-66997] - Western blot analysis on E.coli cell lysates using anti- NusA Mouse mAb

Western Blot: NusA Antibody (A6-E7) [NBP2-66997] - Analysis on E.coli cell lysate using anti- NusA Mouse mAb.

Product Discontinued

NusA Antibody (A6-E7) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full length protein of Escherichia coli NusA. (SwissProt: P0AFF6 Escherichia coli)
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:2000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for NusA Antibody (A6-E7)

• ECK3158
• JW3158
• Transcription termination/antitermination L factor

Background

NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes. Recombinant NusA was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.