Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

SDS-Page: Recombinant E. coli Nucleoside Diphosphate Kinase His Protein [NBP2-75953] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Ec
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant E. coli Nucleoside Diphosphate Kinase His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-143 of E. coli Nucleoside Diphosphate Kinase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

• EC 2.7.4.6
• Multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase
• NDK
• Nucleoside 5'-diphosphate phosphotransferase
• Nucleoside diphosphokinase

Background

ndk, also known as Nucleoside diphosphate kinase, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP. Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.