Nucleocapsid Antibody (1051436) [Alexa Fluor® 488]

• Applications: Flow
• Clone: 1051436
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Nucleocapsid Antibody (1051436) [Alexa Fluor® 488] Summary

• Immunogen: Sf-21 (baculovirus)-derived hcov-oc43 Nucleocapsid
  Met1-Ile448
  Accession # YP_009555245.1
• Specificity: Detects HCoV-OC43 Nucleocapsid in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV‑OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).

1. St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
2. Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
3. Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
4. Hurst, K.R. et al. (2009) J. Virol. 83:7221.
5. Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
6. Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
7. Mourez, T. et al. (2007) J. Virol Methods. 139:175.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.