Nuclease/Nuc A DuoSet ELISA, 5 Plate

NucA nuclease

• Applications: ELISA
• Conjugate: Biotin

Nuclease/Nuc A DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nuclease/Nuc A DuoSet ELISA, 5 Plate

• DcD
• NucA nuclease

Background

Serratia marcescens endonuclease, is a non-specific nuclease that degrades both single- and double-stranded nucleic acids, including DNA and RNA, but exhibits no proteolytic activity (1); therefore, it is ideal for the removal of nucleic acid contaminants from protein samples and applications where complete digestion of nucleic acids is desirable. NucA is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is found to be 8.0-9.2. It hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids to produce 5'-monophosphate oligonucleotides of 3-8 bases in length (2). The active enzyme is a homodimer with two disulfide bonds in each monomer that are crucial to the enzyme activity and stability (3). The absolute activity of the recombinant enzyme is measured by a phosphatase coupled assay (4), where the 5'-phosphate of oligosaccharides generated by the enzyme is further released by non-specific alkaline phosphatase and quantitated by Malachite reagents (5).

Benzonase is a registered trademark of Merck KGaA Corp.

1. Benedik, MJ and Strych, U. (1998) FEMS Microbiol Lett. 165:1.
2. Nestle, M, et al. (1999) J. Biol. Chem. 274:825.
3. Ball, T.K. et al. (1992) Nucleic Acids Res. 20:4971.
4. Wu, Z.L. et al. (2011) Glycobiology 21:727.
5. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.