NSP10 Antibody (1049919) [Alexa Fluor® 647]

• Reactivity: V
• Applications: WB, ICC/IF
• Clone: 1049919
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

NSP10 Antibody (1049919) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived SARS-CoV-2 NSP10 protein
  Ala1-Gln139
  Accession # YP_009725306.1
• Specificity: Detects SARS-CoV-2 NSP10 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NSP10 Antibody (1049919) [Alexa Fluor® 647]

• NSP10

Background

Non-structural protein 10 (NSP10), is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP10 is a small 139 amino acid protein that forms independent dodecameric structures composed of four identical trimers where each monomer is capable of binding two zinc ions (2, 3) that are coordinated uniquely and has exposed basic residues on the protein's surface consequently allowing RNA-binding (3). The NSP10 sequence is conserved across coronaviruses (4) and is thought to serve as an important multifunctional cofactor in viral replication. NSP10 was shown to interact with other NSP proteins including NSP14 and NSP16 (5). The N-terminus of NSP10 has been shown to interact with NSP14 and proposed to stabilize and regulate the 3'-5' exoribonuclease domain activity while not directly impacting the methyltransferase activity of NSP14 (6,7). A similar region of NSP10 interacts with NSP16; this interaction is required for stabilization of NSP16 and stimulation of its 2'-O-methyltransferase activity (4,7). Consequently, NSP10 is critically involved in the formation of functional viral mRNA capping machinery in coronaviruses and a potential target to reduce viral replication and pathogenesis (8,9). 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.