| Description | The NQO1-ARE/LUCPoter(TM) reporter cell line is designed to monitor the induction of ARE and can be used for screening of agonists, antagonists or signaling inhibitors of ARE induction (Nrf2 activity) as well as for studying the Keap1/Nrf2 signaling pathways.
Contents: 3~4 x 10^6 cells Biosafety Level: 2 |
| Immunogen | The NQO1-ARE/LUCPorter(TM) reporter cell line is a stably transfected MCF7 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the antioxidant response element (ARE) derived from the NAD(P)H: quinone oxidoreductase 1 (NQO1) promoter. NQO1 is a flavoprotein that catalyzes two-electron reductive metabolism and detoxification of quinones, protecting cells against quinone-induced oxidative stress and neoplasia. The NQO1 gene promoter contains multiple AREs which regulate induction and expression of NQO1 in response to antioxidants and xenobiotics. Activation of the NQO1 gene transcription through the AREs is primarily regulated by the Keap1-Nrf2 pathway which is the major regulator of cytoprotective responses to endogenous and exogenous stresses caused by reactive oxygen species and electrophiles. The NQO1-ARE/ LUCPorter(TM) reporter cell line can be activated by dimethyl fumarate (DMF), one of the Nrf2 inducers (Scannevin, et al. 2012, J Pharmacol Exp Ther, 341:274), as also shown in Figure 1, in which DMF activated the cell line in a dose response manner (Figure 1). |
| Selection Agent | Puromycin at 1 ug/ml |
| Growth Properties | Adherent Morphology : Epithelial |
| RCL Type | Luciferase - (LUCPorter™) |
| Host | MCF7 |
| Storage | Store in gas phase of liquid nitrogen. |
| Reconstitution Instructions | Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin (Note: The selection agents for this cell line is puromycin at 1 ug/ml). |
