![Immunocytochemistry/Immunofluorescence: npp-9 Antibody [48610002] - This image is specific to animal number SDQ4083 Wild-type (N2) mixed-stage embryos; freeze-thaw MeOH preparation; 1% formaldehyde fixation Primary antibodies: 1:1000 SDQ4083; 1:400 (anti-NPC control) Secondary antibodies: 1:200 anti-rabbit Alexa 488; 1:100 anti-mouse Texas Red DAPI staining](http://images.novusbio.com/fullsize/npp-9-Antibody-Immunocytochemistry-Immunofluorescence-48610002-img0004.jpg "Immunocytochemistry/Immunofluorescence: npp-9 Antibody [48610002] - This image is specific to animal number SDQ4083 Wild-type (N2) mixed-stage embryos; freeze-thaw MeOH preparation; 1% formaldehyde fixation Primary antibodies: 1:1000 SDQ4083; 1:400 (anti-NPC control) Secondary antibodies: 1:200 anti-rabbit Alexa 488; 1:100 anti-mouse Texas Red DAPI staining")
| Immunogen | In vivo generated recominant protein fragment |
| Epitope | MNSLIPPPTSEQQMNMFRLRDKMSLLNAEFLKVINGYFTEKNHYDFSGTMKSYMDHVAQLKQIYKVDDDVAADMTVPRRTENSSESSGETVAPRKIAKAV |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product is useful for ELISA and for Immunofluorescence. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using 48610002 | Applications | Species |
|---|---|---|
| Thomas L, Taleb Ismail B, Askjaer P, Seydoux G Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly The EMBO journal 2023-07-03 [PMID: 37254647] (IHC-WhMt, C. elegans) Details: Dilution 1:250 |
IHC-WhMt | C. elegans |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: npp-9 Antibody [48610002] - This image is specific to animal number SDQ3854 Wild-type (N2) mixed-stage embryos; freeze-thaw MeOH preparation; 1% formaldehyde fixation Primary antibodies: 1:1000 SDQ3854; 1:400 mAb414 (anti-NPC control) Secondary antibodies: 1:200 anti-rabbit Alexa 488; 1:100 anti-mouse Texas Red DAPI staining](http://images.novusbio.com/fullsize/npp-9-Antibody-Immunocytochemistry-Immunofluorescence-48610002-img0003.jpg "Immunocytochemistry/Immunofluorescence: npp-9 Antibody [48610002] - This image is specific to animal number SDQ3854 Wild-type (N2) mixed-stage embryos; freeze-thaw MeOH preparation; 1% formaldehyde fixation Primary antibodies: 1:1000 SDQ3854; 1:400 mAb414 (anti-NPC control) Secondary antibodies: 1:200 anti-rabbit Alexa 488; 1:100 anti-mouse Texas Red DAPI staining")