NovaLume Atto Chemiluminescent Substrate (AP)

Product Discontinued

NovaLume Atto Chemiluminescent Substrate (AP) Summary

• Description: NovaLume Atto Chemiluminescent Substrate (AP) is a ready-to-use stabilized 1, 2-dioxetane substrate.
  
  Features:
• Extreme sensitivity, from low nano to low attogram level detection of alkaline phosphatase (AP)
• Recommended for a wide range of AP applications including blotting (Western Blots, Southern blots), and for detection of alkaline phosphatase-labeled nucleotide and protein probes in solution and on solid supports such as ELISAs
• 100X more sensitive than competitor products
• Highest signal to noise ratios
• High stability plus a 24 month shelf life

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: ELISA: Optimal detection may be obtained from 15 minutes to 120 minutes after contacting the substrate with AP enzyme. MEMBRANES: Blotting conditions should be optimized for each assay system. Visualization is by-ray film or a CCD imaging system. Peak signal may be obtained approximately 30 minutes after substrate contact, which is maintained for several hours.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

NovaLume Atto Chemiluminescent Substrate (AP) shelf life is 24 months when stored in the dark at 2C to 8C. Keep container tightly closed. Store away from heat and light. Detection and analysis requires a chemiluminometer (for ELISA) or imaging system (for Blot). The substrate is supplied as a single bottle reagent, and can be used with various high quality toxin-free blocking buffers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for NovaLume Atto Chemiluminescent Substrate (AP) (NBP2-61914)(4)

Publications using NBP2-61914

• Jandick N, Miller C CREATION AND CHARACTERIZATION OF A RECOMBINANT MAMMALIAN ORTHOREOVIRUS EXPRESSING HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 PEPTIDES bioRxiv 2023-06-13 [PMID: 37634292]
• Dhar D, Mehanovic S, Moss W, Miller C Sequences at gene segment termini inclusive of untranslated regions and partial open reading frames play a critical role in mammalian orthoreovirus S gene packaging bioRxiv 2023-05-27 [PMID: 37292944] (WB)
• Jandick NA, Kirner N, Miller CL Mammalian orthoreovirus infection in human epidermal growth factor receptor 2 positive (HER2+) breast cancer cells bioRxiv : the preprint server for biology 2023-05-10 [PMID: 37214868] (WB)
• Bussiere LD Examination of viral infection and host response to define therapeutic potential of oncolytic mammalian orthoreovirus Thesis 2020-01-01