NOS Activity Assay Kit (Colorimetric) Summary
Description |
Nitric Oxide Synthase Assay Kit is a quantitative colorimetric determination of nitric oxide synthase activity at 540 nm. |
Standard Curve Range |
0.25 to 25 U/L |
Sensitivity |
0.25 U/L |
Assay Type |
Colorimetric |
Sample Volume |
25 uL |
Kit Type |
Assay Kit (Colorimetric) |
Applications/Dilutions
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Substrate
- Standard
- ZnSO4
- Assay Buffer
- GDH
- NaOH
- Reagent B
- Reagent A
- Reagent C
- Reagent D (Dried)
- Reagent E
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
NOS Activity Assay Kit is a method for detecting nitric oxide synthase activity. This is an enzymatic activity assay. Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Nitric Oxide Synthase Activity Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is reduced to as short as 40 min.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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