NOS Activity Assay Kit (Colorimetric)

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Product Details

Summary
Reactivity Mu, PaSpecies Glossary
Applications Func
Suitable Sample Type
Biological Samples
Standard Curve Range
0.25 to 25 U/L
Sensitivity
0.25 U/L

Order Details

NOS Activity Assay Kit (Colorimetric) Summary

Description
Nitric Oxide Synthase Assay Kit is a quantitative colorimetric determination of nitric oxide synthase activity at 540 nm.
Standard Curve Range
0.25 to 25 U/L
Sensitivity
0.25 U/L
Assay Type
Colorimetric
Sample Volume
25 uL
Kit Type
Assay Kit (Colorimetric)

Applications/Dilutions

Publications
Read Publications using KA1634.

Reactivity Notes

Reacts with Cercaria.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Substrate
  2. Standard
  3. ZnSO4
  4. Assay Buffer
  5. GDH
  6. NaOH
  7. Reagent B
  8. Reagent A
  9. Reagent C
  10. Reagent D (Dried)
  11. Reagent E

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

NOS Activity Assay Kit is a method for detecting nitric oxide synthase activity. This is an enzymatic activity assay. Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Nitric Oxide Synthase Activity Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is reduced to as short as 40 min.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for NOS Activity Kit (KA1634)(2)

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Other Available Formats

Additional NOS Activity Products

Array KA1634

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