NOS Activity Assay Kit (Colorimetric)

• Reactivity: Mu, Pa
• Applications: Func
• Suitable Sample Type: Biological Samples
• Standard Curve Range: 0.25 to 25 U/L
• Sensitivity: 0.25 U/L

NOS Activity Assay Kit (Colorimetric) Summary

• Description: Nitric Oxide Synthase Assay Kit is a quantitative colorimetric determination of nitric oxide synthase activity at 540 nm.
• Standard Curve Range: 0.25 to 25 U/L
• Sensitivity: 0.25 U/L
• Assay Type: Colorimetric
• Sample Volume: 25 uL
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Functional

Reactivity Notes

Reacts with Cercaria.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

NOS Activity Assay Kit is a method for detecting nitric oxide synthase activity. This is an enzymatic activity assay. Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Nitric Oxide Synthase Activity Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is reduced to as short as 40 min.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for NOS Activity Kit (KA1634)(2)

Publications using KA1634

• Zhang QL, Yang JJ, Zhang HS. et al. Carvedilol (CAR) combined with carnosic acid (CAA) attenuates doxorubicin-induced cardiotoxicity by suppressing excessive oxidative stress, inflammation, apoptosis and autophagy. Biomed Pharmacother Nov 2 2018 [PMID: 30396094]
• Wang W, Qin Z, Zhu D et al. Synthesis, bioactivity evaluation and toxicity assessment of novel salicylanilide ester derivatives as cercaricides against Schistosoma japonicum and molluscicides against Oncomelania hupensis. Antimicrob Agents Chemother. 2015 Oct 26 [PMID: 26503661]