Normal Goat Serum

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Product Details

Summary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IHC, IHC-P, IP
Host
Goat
Concentration
LYOPH

Order Details

Normal Goat Serum Summary

Specificity
Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Host
Goat

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
Application Notes
Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the secondary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26004262)
Publications
Read Publications using
NBP2-23475 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20 and 0.01% (w/v) Gentamicin Sulfate
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Reconstitution Instructions
Reconstitute in 10 ml with deionized water.

Alternate Names for Normal Goat Serum

  • 10% NGS
  • blocking goat serum
  • blocking grade
  • NGS

Background

Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Normal Goat Serum (NBP2-23475)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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