Normal Goat Serum

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Product Details

Summary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Host
Goat
Concentration
LYOPH

Order Details

Normal Goat Serum Summary

Description
Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Host
Goat
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
This product has been tested by SDS-PAGE and is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 26004262)
Publications
Read Publications using NBP2-23475.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide, 0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute in 10 ml with deionized water.

Alternate Names for Normal Goat Serum

  • 10% NGS
  • blocking goat serum
  • blocking grade
  • NGS

Background

Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Normal Goat Serum (NBP2-23475)(13)

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC-Fr
(2)
IHC-P
(3)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-23475 Applications Species
Gawish R, Maier B, Obermayer G et al. A neutrophil-B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4 eLife 2022-09-30 [PMID: 36178806]
Bourget C, Adams KV, Morshead CM Reduced microglia activation following metformin administration or microglia ablation is sufficient to prevent functional deficits in a mouse model of neonatal stroke Journal of neuroinflammation 2022-06-15 [PMID: 35705953] (IHC-Fr) IHC-Fr
Pyrshev K, Khayyat NH, Stavniichuk A et al. ClC-K2 Cl- channel allows identification of A- and B-type of intercalated cells in split-opened collecting ducts FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-05-01 [PMID: 35349181] (IHC-Fr) IHC-Fr
Niu F, Han P, Zhang J et al. The m(6)A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells eLife 2022-02-18 [PMID: 35179492]
Kasper M, Ellenbogen B, Hardy R et al. Development of a magnetically aligned regenerative tissue-engineered electronic nerve interface for peripheral nerve applications Biomaterials 2021-12-01 [PMID: 34717196]
Zhu LY, Yu LM, Zhang WH et al. Aging Induced p53/p21 in Genioglossus Muscle Stem Cells and Enhanced Upper Airway Injury Stem Cells Int 2020-03-04 [PMID: 32190060] (IHC-P, IF/IHC) IHC-P, IF/IHC
Lacko CS, Singh I, Wall MA et al. Magnetic particle templating of hydrogels: engineering naturally derived hydrogel scaffolds with 3D aligned microarchitecture for nerve repair Journal of Neural Engineering 2020-02-12 [PMID: 31577998]
Jiang S, Hao Z, Li X et al. Ketamine destabilizes growth of dendritic spines in developing hippocampal neurons in vitro via a Rho-dependent mechanism Mol Med Rep 2018-10-02 [PMID: 30280188] (ICC/IF) ICC/IF
Amini P, Stojkov D, Felser A et al. Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production Nat Commun 2018-07-27 [PMID: 30054480]
Li Y, He X, Li J et al. Proliferation and differentiation of direct co-culture of bone marrow mesenchymal stem cells and pigmented cells from the ciliary margin Mol Med Rep 2017-01-01 [PMID: 28440470] (ICC/IF) ICC/IF
Show All 13 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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