Norepinephrine ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA, Quantification
Suitable Sample Type
Biological Fluid
Standard Curve Range
0.2 to 32 ng/mL
Sensitivity
0.1 ng/mL x C*
C* = Correction factor

Order Details

Norepinephrine ELISA Kit (Colorimetric) Summary

Description
Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Norepinephrine in biological fluids.
Standard Curve Range
0.2 to 32 ng/mL
Sensitivity
0.1 ng/mL x C*
C* = Correction factor
Assay Type
Quantification
Intra-Assay
CV% < 15.6 %
Spike Recovery
Human EDTA-Plasma: 116.5%, Cell Culture Medium: 96.7%
Sample Volume
1-750 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Useful for quantification.
Publications
Read Publications using
KA3836 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Adjustment Buffer
  2. Adhesive Foil
  3. Acylation Reagent
  4. Coenzyme
  5. Noradrenaline Antiserum
  6. Noradrenaline Microtiter Strips
  7. Hydrochloric Acid
  8. Microtiter Plate
  9. Extraction Plate
  10. Enzyme
  11. Enzyme Conjugate
  12. Substrate
  13. Stop Solution
  14. TE Buffer
  15. Wash Buffer Concentrate (50X)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Norepinephrine ELISA Kit (Colorimetric)

  • Arterenol
  • Levarterenol
  • L-Noradrenaline
  • L-Norepinephrine
  • noradrenaline

Background

Noradrenaline (Norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Norepinephrine ELISA Kit (KA3836)(4)

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