Mouse Norepinephrine ELISA Kit

Product Discontinued

Mouse Norepinephrine ELISA Kit Summary

• Standard Curve Range: 20 to 339 ng/mL urine, 318 to 2436 pg/mL plasma
• Sensitivity: 1.5 ng/mL urine, 50 pg/mL plasma
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 11.75%
• Intra-Assay: CV% < 12.95%
• Spike Recovery: Urine: 109%, Plasma: 97%
• Sample Volume: Urine: 10 uL, Plasma: 300 uL
• Kit Type: ELISA Kit

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Norepinephrine in plasma and urine. Use in ELISA reported in scientific literature (PMID 27558934).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27558934).

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse Norepinephrine ELISA Kit

• Arterenol
• Levarterenol
• L-Noradrenaline
• L-Norepinephrine
• noradrenaline

Background

Noradrenaline (norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Norepinephrine ELISA Kit (KA1891)(19)

Publications using KA1891

• Alamri HS Talbinah (Barley Porridge): neurotransmitters modulatory effect and antidepressant-like action in experimentally depressed rats European review for medical and pharmacological sciences 2022-01-01 [PMID: 35048996] (ELISA)
• Perego M, Tyurin VA, Tyurina YY et al. Reactivation of dormant tumor cells by modified lipids derived from stress-activated neutrophils Science translational medicine 2020-12-02 [PMID: 33268511] (ELISA, Mouse)
• Wang D, Wu Y, Chen Y et al. Focal selective chemo-ablation of spinal cardiac afferent nerve by resiniferatoxin protects the heart from pressure overload-induced hypertrophy. Biomed Pharmacother 2018-11-03 [PMID: 30399572]
• Ikegami R, Shimizu I, Sato T et al. Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity. Cell Rep 2018-09-11 [PMID: 30208310]
• Fan SM, Chang YT, Chen CL et al. External light activates hair follicle stem cells through eyes via an ipRGC-SCN-sympathetic neural pathway. Proc Natl Acad Sci U S A 2018-06-29 [PMID: 29959210]
• Ishigaki S, Ohashi N, Matsuyama T et al. Melatonin ameliorates intrarenal renin-angiotensin system in a 5/6 nephrectomy rat model. Clin Exp Nephrol 2017-11-20 [PMID: 29159527]
• Chalermkitpanit P, Thonnagith A, Engsusophon P et al. Noradrenaline, Serotonin, GABA, and Glycine in Cerebrospinal Fluid during Labor Pain: A Cross-Sectional Prospective Study. Pain Res Manag 2017-06-18 [PMID: 28701860]
• Gao J, Xu X, Ying Z et al. Post-Effect of Air Quality Improvement on Biomarkers for Systemic Inflammation and Microparticles in Asthma Patients After the 2008 Beijing Olympic Games: a Pilot Study. Inflammation 2017-04-25 [PMID: 28444548]
• Bassi GS, Dias DP, Franchin M et al. Modulation of Experimental Arthritis by Vagal Sensory and Central Brain Stimulation. Brain Behav Immun 2017-04-06 [PMID: 28392428]
• Liu Z, Zhou Z, Jiang Q et al. The neuroendocrine immunomodulatory axis-like pathway mediated by circulating haemocytes in pacific oyster Crassostrea gigas. Open Biol 2017-01-01 [PMID: 28077596]
• Xiao Y, Xia T, Yu J et al. Knockout of inositol-requiring enzyme 1alpha in pro-opiomelanocortin neurons decreases fat mass via increasing energy expenditure. Open Biol. 2016-08-01 [PMID: 27558934] (ELISA, Mouse)
• Lenis YY, Wang X, Tang W et al. Effects of agmatine on secretion of interferon tau and catecholamines and expression of genes related to production of polyamines by ovine trophectoderm cells. Amino Acids 2016-04-13 [PMID: 27074718]
• Kota DJ, Prabhakara KS, van Brummen AJ et al. Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain Injury. Stem Cells Transl Med 2015-11-19 [PMID: 26586775]
• Masson GS, Nair AR, Dange RB et al. Toll-like receptor 4 promotes autonomic dysfunction, inflammation and microglia activation in the hypothalamic paraventricular nucleus: role of endoplasmic reticulum stress. PLoS One. 2015-05-26 [PMID: 25811788]
• Jiang Q, Zhou Z, Wang L et al. Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response. Sci Rep. 2014-11-07 [PMID: 25376551]
• Sun D, Eirin A, Zhu XY et al. Experimental coronary artery stenosis accelerates kidney damage in renovascular hypertensive swine. Kidney Int. 2014-10-22 [PMID: 25337776]
• Ruan HB, Dietrich MO, Liu ZW et al. O-GlcNAc Transferase Enables AgRP Neurons to Suppress Browning of White Fat. Cell. 2014-09-02 [PMID: 25303527]
• Dange RB, Agarwal D, Masson GS et al. Central blockade of TLR4 improves cardiac function and attenuates myocardial inflammation in angiotensin II-induced hypertension. Cardiovasc Res. 2014-03-25 [PMID: 24667851]
• Ying Z, Xu X, Bai Y et al. Long-Term Exposure to Concentrated Ambient PM2.5 Increases Mouse Blood Pressure through Abnormal Activation of the Sympathetic Nervous System: A Role for Hypothalamic Inflammation. Environ Health Perspect. 2013-11-13 [PMID: 24240275]

FAQs for Norepinephrine ELISA Kit (KA1891). (Showing 1 - 6 of 6 FAQs).

1. I would like to measure adrenergic receptors (alpha and beta) in lympocytes and platelets, respectively; can you help? I would like to measure beta and alpha adrenergic receptors in rat lympocytes and platelets. Please email me quote for 125iodocyanopindolol, 125iodopindolol 3H prazosin 3H rauvolscine and 3H yohimine. Quotes for ELISA kits to measure insulin, in 250 samples, ELISA kit to measure norephinephrine.
   • We do have an ELISA kit for norepinephrine (catalog # KA1891). Unfortunately, we do not have experience in (or knowledge of) measuring the density of adrenergic receptors; we suggest you consult the literature for suitable methods. If you then decide you would like to order a particular product from us, we would love to hear from you again.
2. I want to measure noradrenaline level in mouse plasma. please let me know whether the product (KA1891) is reactive to mouse.
   • The norepinephrine ELISA kit KA1891 is a product we distribute for a Taiwanese company called Abnova. This particular kit is an enzyme immunoassay for the quantitative determination of Norepinephrine in plasma and urine. Since norepinephrine is a hormone and not a protein, the molecule is species independent. Therefore, this kit will meet your needs. I will include a link to the protocol included with the kit for your reference
3. Will this work for bovine norepi?
   • For kit KA1891, since the ELISA target is a neurotransmitter and hormone, it will not be species specific, but specific for the compound itself. Therefore, this kit can be used with a variety of species. The only restriction for this kit will be the sample type. Thus far, it has only been tested with Plasma and Urine.
4. Can I dilute the sample with NaOH to increase the pH?
   • Yes, pH adjustment by addition of NaOH can be an option in this case. A pH around 3 or 4 would be ideal because that would be normal for urine. The pH of the standards is around 1, so this might also work. But it might denature the proteins of the urine, so a higher pH would be better.
5. Can this kit be used with rat serum/plasma?
   • Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
6. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.