Nitrotyrosine ELISA Kit (Colorimetric)

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Diagram of the Triplicate Sample Plate Format

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) - NBP2-59717. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM.

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Diagram of the Competitive ELISA

Product Discontinued

Nitrotyrosine ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 62.5 - 8000 nM
• Sensitivity: 50 nM
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < <15%
• Intra-Assay: CV% < 10%.
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: ELISA kit used to quantitate Nitrotyrosine in samples.
  No of Samples: 38 samples in duplicate
  Incubation Time: 1 hour

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Nitrotyrosine ELISA Kit (Colorimetric)

• Nitrotyrosine

Background

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Nitrotyrosine ELISA Kit (NBP2-59717). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.