Nitrotyrosine ELISA Kit (Colorimetric)

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Diagram of the Triplicate Sample Plate Format

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) - NBP2-59717. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM.

ELISA: Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-59717] - Diagram of the Competitive ELISA

Product Discontinued

Nitrotyrosine ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 62.5 - 8000 nM
• Sensitivity: 50 nM
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < <15%
• Intra-Assay: CV% < 10%.
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: ELISA kit used to quantitate Nitrotyrosine in samples.
  No of Samples: 38 samples in duplicate
  Incubation Time: 1 hour

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Nitrotyrosine ELISA Kit (Colorimetric)

• Nitrotyrosine

Background

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Nitrotyrosine ELISA Kit (NBP2-59717). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.