NIH 3T3 Nuclear Histone Prep Cell Lysate

Product Discontinued

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01 mg per lane.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Triton Extraction Buffer (1 X PBS containing 0.5% Triton X 100 (v/v) and a cocktail of phosphatase and protease inhibitors) with 1 X Lithium Dodecyl Sulfate (LDS) Sample Buffer at pH 8.4. BME has not been added.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Bone/Cartilage
• Subcellular Fraction: Nuclear Histone Prep

Background

NIH3T3 cells are mouse embryonic fibroblast cells that are derived from Swiss mouse embryonic tissue. 3T3 is an abbreviation of 3-day transfer, inoculum 3 x 10^5 cells. NIH3T3 cells are used primarily in the cultivation of keratinocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.