NEUROBIOTIN® [351 Tracer]

NEUROBIOTIN(R) [351 Tracer] [SP-1155-2-NB]

• Conjugate: 351 Tracer

NEUROBIOTIN® [351 Tracer] Summary

• Description: NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.
  
  Features
• Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
• Biotin label with a biotinidase-resistant linkage
• Fixable primary amine
• Used for visualizing neural architecture and for the identification of gap junction coupling
• Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
• Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
• Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods


Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels
• Better solubility
• More efficiently iontophoresed
• Remains in cell longer
• Non-toxic
• Can be fixed with formalin or glutaraldehyde

Applications/Dilutions

• Application Notes: Once in solution, store frozen. Usage NEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: a biotin label with a biotinidase-resistant linker, a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor 350, a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde..

Packaging, Storage & Formulations

• Storage: Desiccate at +4C

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.