NEUROBIOTIN® [488 Tracer]

NEUROBIOTIN(R) [488 Tracer] [SP-1125-2-NB]

NEUROBIOTIN(R) [488 Tracer] [SP-1125-2-NB]

NEUROBIOTIN(R) [488 Tracer] [SP-1125-2-NB]

Product Discontinued

NEUROBIOTIN® [488 Tracer] Summary

• Description: NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.
  
  Features
• Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
• Biotin label with a biotinidase-resistant linkage
• Fixable primary amine
• Used for visualizing neural architecture and for the identification of gap junction coupling
• Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
• Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading


Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels
• Better solubility
• More efficiently iontophoresed
• Remains in cell longer
• Non-toxic
• Can be fixed with formalin or glutaraldehyde

Applications/Dilutions

• Application Notes: Once in solution, store frozen. NEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO.

Packaging, Storage & Formulations

• Storage: Desiccate at +4C

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.