Neomycin phosphotransferase II Antibody (4B4D1)

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1) [NB110-60487] - Figure 1: Western blot analysis using anti-human NPT monoclonal antibody against recombinant NPT expressed in E. Coli.

• Reactivity: All-NA, Ba
• Applications: WB, ELISA
• Clone: 4B4D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Neomycin phosphotransferase II Antibody (4B4D1) Summary

• Immunogen: Purified recombinant fragment of Neomycin phosphotransferase II expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000

Reactivity Notes

Transfected or transformed cells / organisms (plants, animals) carrying NPTII gene encoding neomycin phosphotransferase II enzyme. Escherichia coli (E. coli).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Neomycin phosphotransferase II Antibody (4B4D1)

• NPT2
• NPTII

Background

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.