Recombinant Bacteria NAD Nucleotidase Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant Bacteria NAD Nucleotidase Protein Summary

• Description: Full-length NAD 5'-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa.
  
  Source: E. coli

  Uniprot ID: Q4QNY4

• Details of Functionality: With AMP: 856 4 nmol of Ade/min/mg of protein
  With NMN: 110,3 nmol of NR/min/mg of protein
  With NAD: 5 4 nmol of Ade/min per mg of protein | 5 3 nmol of NR/min/mg of protein
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: nadN
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

H. influenzae

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 5mM KH2PO4, pH 6.8, 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant Bacteria NAD Nucleotidase Protein

• nadN

Background

NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: nadN
• Uniprot:
  • Q4QNY4(Bacteria)