Myofibroblast Marker Antibody (PR 2D3) - Azide and BSA Free

Western Blot: Myofibroblast Marker Antibody (PR 2D3) - Azide and BSA Free [NBP3-08768] - Western Blot Analysis of A431, MCF-7 & HeLa cell lysates using Myofibroblast Marker Mouse Monoclonal Antibody (PR 2D3).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PR 2D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Myofibroblast Marker Antibody (PR 2D3) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07745).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Crude homogenate of normal human colorectal mucosa
• Localization: Cell membrane component of cells in the pericrypt sheath; with smooth muscle cells and myofibroblasts.
• Marker: Myofibroblast Marker
• Specificity: Myofibroblasts are a unique group of smooth muscle-like fibroblasts that play an important role in oncogenesis, inflammation, repair, wound contraction and fibrosis. Like smooth muscle (SM) cells, myofibroblasts contain microfilament bundles and express -SM Actin, the Actin isoform that is present in myoepithelial cells and SM cells and especially abundant in vascular SM cells. Myofibroblasts secrete inflammatory and anti-inflammatory cytokines, chemokines, growth factors and lipid and gaseous inflammatory mediators, as well as extracellular matrix proteins and proteases in most organs and tissues. Besides being temporarily present following tissue injuries and fibrocontractive diseases, myofibroblasts are also present under normal conditions in regions such as the skin, pulmonary septa and periodontal ligaments. Stem cell factor and platelet-derived growth factor (PDGF) are two secreted proteins responsible for differentiating myofibroblasts from embryological stem cells. PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath; with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.