Mycoplasma pneumoniae ELISA Kit (Colorimetric) Summary
| Description |
Mycoplasma pneumoniae IgA ELISA Kit is a solid phase enzyme immunoassay for the semiquantitative measurement of IgA autoantibodies against Mycoplasma pneumoniae. |
| Assay Type |
solid phase enzyme-linked immunosorbent assay |
| Inter-Assay |
CV% < 8.65% |
| Intra-Assay |
CV% < 4.25% |
| Spike Recovery |
94% to 115% |
| Sample Volume |
100 uL (1:100 prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- 0.2 mL Anti-human IgA antibodies labeled with horseraddish peroxidase 101x concentrated (Px-conjugate)
- 1)ready to use
- 1.3 mL Calibrator r.t.u.1
- 1.3 mL Negative control serum r.t.u.
- 1.3 mL Positive control serum r.t.u.
- 125 mL Dilution buffer (DB) r.t.u.
- 125 mL Wash buffer 10x concentrated
- 15 mL Chromogenic substrate (TMB substrate) r.t.u.
- 15 mL Stop solution r.t.u.
- Certificate of quality
- ELISA break-away strips (yellow) coated with specific antigen
- Instruction manual
- Sealable pouch for unused strips
- Wash buffer, Chromogenic substrate (TMB substrate) r.t.u and Dilution buffer are intended only forMycoplasma pneumoniae IgA ELISA Kit and THEY ARE NOT COMPATIBLE with other ELISA kits
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mycoplasma pneumoniae ELISA Kit (Colorimetric)
Background
Since it was first recognized in the early 1960's, mycoplasma mastitis has been reported in many dairy farms throughout the United States. Mycoplasma are opportunistic invaders and can cause joint infections, pneumonia, metritis, and mastitis. Several species of Mycoplasma have been found in dairy cattle, but by far the most prevalent is Mycoplasma bovis, which is responsible for more than half of all reported isolates. This specie also causes the most severe infections of any Mycoplasma organism known to cause disease in cattle.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Mycoplasma pneumoniae ELISA Kit (KA1463). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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