Recombinant Mycobacterium Tuberculosis ESAT6 His Protein

SDS-Page: Recombinant Mycobacterium Tuberculosis ESAT6 Protein [NBP1-99045] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Mycobacterium Tuberculosis ESAT6 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-95 of Mycobacterium tuberculosis Mycobacterium Tuberculosis ESAT6
  
  

  Source: Baculovirus

  Amino Acid Sequence: ADPMTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFAHH HHHH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mycobacterium tuberculosis

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mycobacterium Tuberculosis ESAT6 His Protein

• 6 kDa early secretory antigenic target ESXA
• 6 kDa early secretory antigenic target
• ESAT 6
• esaT6
• ESXA

Background

ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Mycobacterium Tuberculosis ESAT6 Recombinant Protein (NBP1-99045)(4)

Publications using NBP1-99045

• Shurygina AP, Zabolotnykh N, Vinogradova T et al. Preclinical Evaluation of TB/FLU-04L-An Intranasal Influenza Vector-Based Boost Vaccine against Tuberculosis International journal of molecular sciences 2023-04-18 [PMID: 37108602] (In vitro assay)
• He J, Fan Y, Shen D et al. Characterization of cytokine profile to distinguish latent tuberculosis from active tuberculosis and healthy controls Cytokine 2020-08-06 [PMID: 32771857] (Human)
• Jang AR, Choi JH, Shin SJ, Park JH. Mycobacterium tuberculosis ESAT6 induces IFN-b gene expression in Macrophages via TLRs-mediated signaling. Cytokine. 2017-10-15 [PMID: 29046251] (Bacteria)
• Koyama Y, Ito T, Hasegawa A et al. Exosomes derived from tumor cells genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy. Biotechnol. Lett. 2016-08-02 [PMID: 27484689] (ICC/IF, Fish)