Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [DyLight 594]

Product Discontinued

Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [DyLight 594] Summary

• Immunogen: Purified protein derivative (PPD) of tuberculin from Mycobacterium tuberculosis cojugatedwith thimerosal N-hydroxysuccinimide ester and adsorbed to aluminum hydroxide gel (4)
• Epitope: Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (5)
• Specificity: Reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of M. tuberculosis and virulent strains of M. bovis. This antibody cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B, and C components of the antigen 85 complex and with MPT51.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [DyLight 594]

• 6 kDa early secretory antigenic target ESXA
• 6 kDa early secretory antigenic target
• ESAT 6
• esaT6
• ESXA

Background

ESAT-6 (6-kDa early secretory antigenic target) from Mycobacterium tuberculosis H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection (2). The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum, and M. szulgai, but not in avirulent M. bovis BCG substrains (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.