Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [Alexa Fluor® 405]

Product Discontinued

Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [Alexa Fluor® 405] Summary

• Immunogen: Purified protein derivative (PPD) of tuberculin from Mycobacterium tuberculosis cojugatedwith thimerosal N-hydroxysuccinimide ester and adsorbed to aluminum hydroxide gel (4)
• Epitope: Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (5)
• Specificity: Reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of M. tuberculosis and virulent strains of M. bovis. This antibody cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B, and C components of the antigen 85 complex and with MPT51.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mycobacterium Tuberculosis ESAT6 Antibody (11 G4) [Alexa Fluor® 405]

• 6 kDa early secretory antigenic target ESXA
• 6 kDa early secretory antigenic target
• ESAT 6
• esaT6
• ESXA

Background

ESAT-6 (6-kDa early secretory antigenic target) from Mycobacterium tuberculosis H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection (2). The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum, and M. szulgai, but not in avirulent M. bovis BCG substrains (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.